3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation

3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation Publisher Pubmed

Safiaghdam H¹ ; Nokhbatolfoghahaei H² ; Farzadmohajeri S^{3, 4} ; Dehghan MM^{3, 4} ; Farajpour H⁵ ; Aminianfar H^{4, 6} ; Bakhtiari Z⁷ ; Jabbari Fakhr M^{4, 8} ; Hosseinzadeh S^{5, 9} ; Khojasteh A^{2, 10}

Show Affiliations

1. Student Research Committee, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4. Institute of Biomedical Research, University of Tehran, Tehran, Iran
5. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
7. Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
8. Department of Tissue Engineering, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran
9. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
10. Department of Cranio-Maxillofacial Surgery/University Hospital, Faculty of Medicine & Health Sciences, University of Antwerp, Antwerp, Belgium

Source: Journal of Biomedical Materials Research - Part A Published:2023

Abstract

Magnesium (Mg) plays an important role in controlling bone apatite structure and density and is a potential bioactive material in repairing critical-sized bone defects. In this study, we aimed to evaluate the effect of adding NanoMgO to polycaprolactone/beta-tricalcium phosphate (PCL/β-TCP) scaffolds on bone regeneration. Novel 3D-printed porous PCL/β-TCP composite scaffolds containing 10% nanoMgO were fabricated by fused deposition modeling (FDM) and compared with PCL/β-TCP (1:1) scaffolds (control). The morphology and physicochemical properties of the scaffolds were characterized by ATR-FTIR, XRD, scanning electron microscope-energy dispersive X-ray analysis (SEM–EDX), transmission-electron-microscopy (TEM), water contact angle, and compressive strength tests and correlated to its cytocompatibility and osteogenic capacity in-vitro. To evaluate in-vivo osteogenic capacity, bone-marrow-derived stem cell (BMSC)-loaded scaffolds were implanted into 8 mm rat critical-sized calvarial defects for 12 weeks. The hydrophilic scaffolds showed 50% porosity (pore size = 504 μm). MgO nanoparticles (91.5 ± 27.6 nm) were homogenously dispersed and did not adversely affect BMSCs' viability and differentiation. Magnesium significantly increased elastic modulus, pH, and degradation. New bone formation (NBF) in Micro-CT was 30.16 ± 0.31% and 23.56 ± 1.76% in PCL/β-TCP/nanoMgO scaffolds with and without BMSCs respectively, and 19.38 ± 2.15% and 15.75 ± 2.24% in PCL/β-TCP scaffolds with and without BMSCs respectively. Angiogenesis was least remarkable in PCL/β-TCP compared with other groups (p <.05). Our results suggest that the PCL/β-TCP/nanoMgO scaffold is a more suitable bone substitute compared to PCL/β-TCP in critical-sized calvarial defects. © 2022 Wiley Periodicals LLC.

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Style	Citing Format
MLA	Safiaghdam H, et al.. "3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation." Journal of Biomedical Materials Research - Part A, vol. 111, no. 3, 2023, pp. 322-339.
APA	Safiaghdam H, Nokhbatolfoghahaei H, Farzadmohajeri S, Dehghan MM, Farajpour H, Aminianfar H, Bakhtiari Z, Jabbari Fakhr M, Hosseinzadeh S, Khojasteh A (2023). 3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation. Journal of Biomedical Materials Research - Part A, 111(3), 322-339.
Chicago	Safiaghdam H, Nokhbatolfoghahaei H, Farzadmohajeri S, Dehghan MM, Farajpour H, Aminianfar H, Bakhtiari Z, Jabbari Fakhr M, Hosseinzadeh S, Khojasteh A. "3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation." Journal of Biomedical Materials Research - Part A 111, no. 3 (2023): 322-339.
Harvard	Safiaghdam H et al. (2023) '3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation', Journal of Biomedical Materials Research - Part A, 111(3), pp. 322-339.
Vancouver	Safiaghdam H, Nokhbatolfoghahaei H, Farzadmohajeri S, Dehghan MM, Farajpour H, Aminianfar H, et al.. 3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation. Journal of Biomedical Materials Research - Part A. 2023;111(3):322-339.
BibTex	@article{ author = {Safiaghdam H and Nokhbatolfoghahaei H and Farzadmohajeri S and Dehghan MM and Farajpour H and Aminianfar H and Bakhtiari Z and Jabbari Fakhr M and Hosseinzadeh S and Khojasteh A}, title = {3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation}, journal = {Journal of Biomedical Materials Research - Part A}, volume = {111}, number = {3}, pages = {322-339}, year = {2023} }
RIS	TY - JOUR AU - Safiaghdam H AU - Nokhbatolfoghahaei H AU - Farzadmohajeri S AU - Dehghan MM AU - Farajpour H AU - Aminianfar H AU - Bakhtiari Z AU - Jabbari Fakhr M AU - Hosseinzadeh S AU - Khojasteh A TI - 3D-Printed Mgo Nanoparticle Loaded Polycaprolactone Β-Tricalcium Phosphate Composite Scaffold for Bone Tissue Engineering Applications: In-Vitro and In-Vivo Evaluation JO - Journal of Biomedical Materials Research - Part A VL - 111 IS - 3 SP - 322 EP - 339 PY - 2023 ER -

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