Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effects of Berberine Hydrochloride on Methamphetamine-Induced Anxiety Behaviors and Relapse in Rats Publisher



Rezaeian L1 ; Kalalianmoghaddam H2 ; Mohseni F3 ; Khaksari M4 ; Rafaiee R5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Addiction Studies, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  2. 2. Addiction Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
  3. 3. Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  4. 4. Department of Physiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  5. 5. Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Source: Iranian Journal of Basic Medical Sciences Published:2020


Abstract

Objective(s): This research aimed at evaluating the effect of berberine hydrochloride on anxiety-related behaviors induced by methamphetamine (METH) in rats, assessing relapse and neuroprotective effects. Materials and Methods: 27 male Wistar rats were randomly assigned into groups of Control, METH-withdrawal (METH addiction and subsequent withdrawal), and METH addiction with berberine hydrochloride oral treatment (100 mg/kg/per day) during the three weeks of withdrawal. Two groups received inhaled METH self-administration for two weeks (up to 10 mg/kg). The elevated plus maze (EPM) test and open field test (OFT) were carried out one day after the last berberine treatment and relapse was assessed by conditional place preference (CPP) test. TUNEL assay and immunofluorescence staining for NF-κB, TLR4, Sirt1, and α-actin expression in the hippocampus were tested. Results: After 3 weeks withdrawal, berberine hydrochloride decreased locomotor activity and reduced anxiety-related behaviors in comparison with the METH-withdrawal group (P<0.001). The obtained results from CPP showed that berberine significantly reduced relapse (P<0.01). Significantly decrease in activation of TLR4, Sirt1, and α-actin in METH-withdrawal group was found and the percentage of TLR4, Sirt1, and α-actin improved in berberine-treated group (P<0.001). A significant activity rise of NF-κB of cells in the METH-withdrawal group was detected compared to berberine-treated and control groups (P<0.001). Conclusion: Treatment with berberine hydrochloride via modulating neuroinflammation may be considered as a potential new medication for the treatment of METH addiction and relapse. The histological assays supported the neuroprotective effects of berberine in the hippocampus. © 2020 Mashhad University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Berberine and Neurodegeneration: A Review of Literature, Pharmacological Reports (2015)
2. Concomitant Abuse of Methadone and Methamphetamine Could Impair Spatial Learning and Memory in Male Rats, Learning and Motivation (2019)
3. Evidence for Possible Role of Melatonin in Reducing Oxidative Stress in Multiple Sclerosis Through Its Effect on Sirt1 and Antioxidant Enzymes, Life Sciences (2016)
4. Effects of Berberine on Leukemia With a Focus on Its Molecular Targets, Anti-Cancer Agents in Medicinal Chemistry (2022)
5. Effect of Berberine on Bcl-2 Expression in Chronic Lymphocytic Leukemia Compared With Healthy Controls, Journal of Mazandaran University of Medical Sciences (2019)
6. Targeting the Tlr4 Signaling Pathway by Polyphenols: A Novel Therapeutic Strategy for Neuroinflammation, Ageing Research Reviews (2017)
7. Effects of Hydroalcoholic Extract of Berberis Integerrima on the Clinical Signs, Hs-Crp, Tnfα, and Esr in Active Rheumatoid Arthritis Patients, Journal of Herbal Medicine (2021)
8. Role of Natural Products for the Treatment of Alzheimer's Disease, European Journal of Pharmacology (2021)
Experts (# of related papers)
View all Related Experts
Mohammad Abdollahi (2)
Nima Rezaei (1)
Other Related Docs
9. Selegiline Acts As Neuroprotective Agent Against Methamphetamine-Prompted Mood and Cognitive Related Behavior and Neurotoxicity in Rats: Involvement of Creb/Bdnf and Akt/Gsk3 Signal Pathways, Iranian Journal of Basic Medical Sciences (2020)
10. Neuroinflammatory Response in Reward-Associated Psychostimulants and Opioids: A Review, Cellular and Molecular Neurobiology (2023)
11. Toll-Like Receptors in Alzheimer's Disease, Journal of Neuroimmunology (2020)
12. Can Berberine Serve As a New Therapy for Parkinson’S Disease?, Neurotoxicity Research (2022)
13. Amantadine Reduces Persistent Fatigue During Post-Acute Withdrawal Phase in Methamphetamine Abstained Individuals: A Randomized Placebo-Controlled Trial, Journal of Substance Use (2018)