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Exosomes From Adipose Tissue-Derived Mesenchymal Stem Cells Induce Regulatory T Cells in Covid‐19 Patients Publisher Pubmed



Motallebnezhad M1 ; Hazrati A2 ; Ghaleh HEG3 ; Jonaidijafari N1 ; Abbaspouraghdam S4 ; Malekpour K5 ; Yousefi M4 ; Kafil HS6 ; Jadidiniaragh F7 ; Roshangar L4 ; Valizadeh H8 ; Izadi M1 ; Ahmadi M4
Authors
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Authors Affiliations
  1. 1. Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  4. 4. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  5. 5. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  6. 6. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  7. 7. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  8. 8. Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran

Source: Iranian Journal of Allergy# Asthma and Immunology Published:2023


Abstract

An imbalance between regulatory T (Treg) and T-helper (Th)-17 cells has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) exert immunomodulatory properties through secreting exosomes. This study aimed to assess the effect of MSC-derived exosomes (MSC-Exo) on the differentiation of peripheral blood mononuclear cells (PBMCs) into Tregs from patients with COVID-19. Exosomes were isolated from adipose tissue–derived MSCs. PBMCs were separated from the whole blood of COVID-19 patients (n=20). Treg frequency was assessed before and 48 hours after treatment of PBMCs with MSC-Exo using flow cytometry. Expression of FOXP3 and cytokine genes, and the concentration of cytokines associated with Tregs, were assessed before and after treatment with MSC-Exo. The frequency of CD4+CD25+CD127- Tregs was significantly higher after treating PBMCs with MSC-Exo (6.695±2.528) compared to before treatment (4.981±2.068). The expressions of transforming growth factor (TGF)-β1, interleukin (IL)-10, and FOXP3 were significantly upregulated in MSC-Exo–treated PBMCs. The concentration of IL‐10 increased significantly after treatment (994.7±543.9 pg/mL) of PBMCs with MSC-Exo compared with before treatment (563.5±408.6 pg/mL). The concentration of TGF-β was significantly higher in the supernatant of PBMCs after treatment with MSC-Exo (477.0±391.1 pg/mL) than PBMCs before treatment (257.7±226.3 pg/mL). MSC-Exo has the potential to raise anti-inflammatory responses by induction of Tregs, potentiating its therapeutic effects in COVID-19. Copyright © 2023 Motallebnezhad et al. Published by Tehran University of Medical Sciences.
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