T Lymphocyte Characteristic Changes Under Serum Cytokine Deviations and Prognostic Factors of Covid-19 in Pregnant Women Publisher



Aminsobahni E^{1, 2} ; Hosseini M³ ; Gholizadeh N⁴ ; Soltanizangbar MS^{5, 6} ; Savari G⁵ ; Motlagh Asghari K⁵ ; Pourlak T⁷ ; Zolfaghari M⁸ ; Chakarikhiavi F⁵ ; Motavalli R^{5, 8} ; Chakarikhiavi A⁹ ; Shekarchi AA¹⁰ ; Mahmoodpoor A¹¹ ; Ahmadian Heris J¹² Show All Authors Show Affiliations
Source: Applied Biochemistry and Biotechnology Published:2023

Abstract

Physiological changes during pregnancy make the individuals more susceptible to severe respiratory diseases. Hence, pregnant women with coronavirus disease 2019 (COVID-19) are likely at a higher risk. We investigated the effects of COVID-19 on T cell response and serum cytokine profile in pregnant patients. Peripheral blood mononuclear cells (PBMCs) of women with COVID-19 were collected during the first trimester of pregnancy, and the percentage of total lymphocytes, as well as CD4 + and CD8 + T cells, was assessed using flow cytometry. The expression of the programmed death-1 (PD-1) marker for exhausted T cells was evaluated. Additionally, the serum samples were provided to evaluate the levels of antiviral and proinflammatory cytokines, as well as laboratory serological tests. Pregnant women with COVID-19 presented lymphopenia with diminished CD4 + and CD8 + T cells. Besides, high expression levels of the PD-1 gene and protein were observed on PBMCs and T cells, respectively, when compared with normal pregnant individuals. Moreover, serum levels of TNF-α, IL-6, IL-1β, and IL-2 receptor were notably enhanced, while IFN-I α/β values were significantly decreased in the patients when compared with controls. Furthermore, hyperlipidemia, hyperglycemia, and hypertension were directly correlated with the disease although serum albumin and vitamin D3 levels adversely affected the viral infection. Our study showed extreme lymphopenia and poor T cell response while elevated values of serum inflammatory cytokines in infected pregnant women. Moreover, a hypertension background or metabolic changes, including hyperlipidemia, hyperglycemia, and vitamin D3 or albumin deficiency, might be promising prognostic factors in pregnant women with COVID-19. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.