Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection

Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection Publisher

Javanmard D¹ ; Najafi M² ; Babaei MR³ ; Karbalaie Niya MH⁴ ; Esghaei M¹ ; Panahi M⁴ ; Tameshkel FS^{4, 5} ; Tavakoli A¹ ; Jazayeri SM^{6, 7} ; Ghaffari H¹ ; Ataeipirkooh A¹ ; Monavari SH¹ ; Bokharaeisalim F^{1, 8}

Show Affiliations

1. Department of Virology, Iran University of Medical Sciences, Tehran, Iran
2. Department of Biochemistry, School of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
3. Department of Interventional Radiology, Firouzgar Hospital, Iran University of Medical Sciences, Tehran, Iran
4. Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran
5. Student Research Committee, Iran University of Medical Sciences, Tehran, IR, Iran
6. Department of Virology, Tehran University of Medical Science, Tehran, Iran
7. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
8. HIV Laboratory of National Center, Deputy of Health, Iran University of Medical Sciences, Tehran, Iran

Source: Infectious Agents and Cancer Published:2020

Abstract

Hepatitis B virus (HBV), along with Hepatitis C virus chronic infection, represents a major risk factor for hepatocellular carcinoma (HCC) development. However, molecular mechanisms involved in the development of HCC are not yet completely understood. Recent studies have indicated that mutations in CTNNB1 gene encoding for β-catenin protein lead to aberrant activation of the Wnt/ β-catenin pathway. The mutations in turn activate several downstream genes, including c-Myc, promoting the neoplastic process. The present study evaluated the mutational profile of the CTNNB1 gene and expression levels of CTNNB1 and c-Myc genes in HBV-related HCC, as well as in cirrhotic and control tissues. Mutational analysis of the β-catenin gene and HBV genotyping were conducted by direct sequencing. Expression of β-catenin and c-Myc genes was assessed using real-time PCR. Among the HCC cases, 18.1% showed missense point mutation in exon 3 of CTNNB1, more frequently in codons 32, 33, 38 and 45. The frequency of mutation in the hotspots of exon 3 was significantly higher in non-viral HCCs (29.4%) rather than HBV-related cases (12.7%, P = 0.021). The expression of β-catenin and c-Myc genes was found upregulated in cirrhotic tissues in association with HBV infection. Mutations at both phosphorylation and neighboring sites were associated with increased activity of the Wnt pathway. The results demonstrated that mutated β-catenin caused activation of the Wnt pathway, but the rate of CTNNB1 gene mutations was not related to HBV infection. HBV factors may deregulate the Wnt pathway by causing epigenetic alterations in the HBV-related HCC. © 2020 The Author(s).

2. Signal Transduction Pathway Mutations in Gastrointestinal (Gi) Cancers: A Systematic Review and Meta-Analysis, Scientific Reports (2020)

3. Potential Role of Mir-214 in Β-Catenin Gene Expression Within Hepatocellular Carcinoma, Molecular Biology Reports (2020)

Experts (# of related papers)

Seyed Mohammad Jazayeri (1)

Style	Citing Format
MLA	Javanmard D, et al.. "Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection." Infectious Agents and Cancer, vol. 15, no. 1, 2020, pp. -.
APA	Javanmard D, Najafi M, Babaei MR, Karbalaie Niya MH, Esghaei M, Panahi M, Tameshkel FS, Tavakoli A, Jazayeri SM, Ghaffari H, Ataeipirkooh A, Monavari SH, Bokharaeisalim F (2020). Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection. Infectious Agents and Cancer, 15(1), -.
Chicago	Javanmard D, Najafi M, Babaei MR, Karbalaie Niya MH, Esghaei M, Panahi M, Tameshkel FS, et al.. "Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection." Infectious Agents and Cancer 15, no. 1 (2020): -.
Harvard	Javanmard D et al. (2020) 'Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection', Infectious Agents and Cancer, 15(1), pp. -.
Vancouver	Javanmard D, Najafi M, Babaei MR, Karbalaie Niya MH, Esghaei M, Panahi M, et al.. Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection. Infectious Agents and Cancer. 2020;15(1):-.
BibTex	@article{ author = {Javanmard D and Najafi M and Babaei MR and Karbalaie Niya MH and Esghaei M and Panahi M and Tameshkel FS and Tavakoli A and Jazayeri SM and Ghaffari H and Ataeipirkooh A and Monavari SH and Bokharaeisalim F}, title = {Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection}, journal = {Infectious Agents and Cancer}, volume = {15}, number = {1}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Javanmard D AU - Najafi M AU - Babaei MR AU - Karbalaie Niya MH AU - Esghaei M AU - Panahi M AU - Tameshkel FS AU - Tavakoli A AU - Jazayeri SM AU - Ghaffari H AU - Ataeipirkooh A AU - Monavari SH AU - Bokharaeisalim F TI - Investigation of Ctnnb1 Gene Mutations and Expression in Hepatocellular Carcinoma and Cirrhosis in Association With Hepatitis B Virus Infection JO - Infectious Agents and Cancer VL - 15 IS - 1 SP - EP - PY - 2020 ER -

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