Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1

Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1 Publisher Pubmed

Kouhkan F¹ ; Mobarra N² ; Soufizomorrod M³ ; Keramati F¹ ; Ali Hosseini Rad SM¹ ; Fathiroudsari M⁴ ; Tavakoli R¹ ; Hajarizadeh A¹ ; Ziaei S^{1, 5} ; Lahmi R⁶ ; Hanif H⁷ ; Soleimani M^{1, 3}

Show Affiliations

1. Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
2. Metabolic Disorders Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3. Department of Hematology, School of Medicine, Tarbiat Modares University, P.O. Box:14115-331, Tehran, Iran
4. National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
5. Department of Basic Sciences, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. Department of Neuroscience, Aging and Stem Cell Research Center, Sanford- Burnham Medical Research Institute, La Jolla, CA, United States
7. Department of Neurosurgery, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Medical Genetics Published:2015

Abstract

Background MicroRNA-129-1 (miR-129-1) seems to behave as a tumour suppressor since its decreased expression is associated with different tumours such as glioblastoma multiforme (GBM). GBM is the most common form of brain tumours originating from glial cells. The impact of miR-129-1 downregulation on GBM pathogenesis has yet to be elucidated. Methods MiR-129-1 was overexpressed in GBM cells, and its effect on proliferation was investigated by cell cycle assay. MiR-129-1 predicted targets (CDK6, IGF1, HDAC2, IGF2BP3 and MAPK1) were also evaluated by western blot and luciferase assay. Results Restoration of miR-129-1 reduced cell proliferation and induced G1 accumulation, significantly. Several functional assays confirmed IGF2BP3, MAPK1 and CDK6 as targets of miR-129-1. Despite the fact that IGF1 expression can be suppressed by miR-129-1, through 30-untranslated region complementary sequence, we could not find any association between IGF1 expression and GBM. MiR-129-1 expression inversely correlates with CDK6, IGF2BP3 and MAPK1 in primary clinical samples. Conclusion This is the first study to propose miR129-1 as a negative regulator of IGF2BP3 and MAPK1 and also a cell cycle arrest inducer in GBM cells. Our data suggests miR-129-1 as a potential tumour suppressor and presents a rationale for the use of miR-129-1 as a novel strategy to improve treatment response in GBM.

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Style	Citing Format
MLA	Kouhkan F, et al.. "Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1." Journal of Medical Genetics, vol. 53, no. 1, 2015, pp. 24-33.
APA	Kouhkan F, Mobarra N, Soufizomorrod M, Keramati F, Ali Hosseini Rad SM, Fathiroudsari M, Tavakoli R, Hajarizadeh A, Ziaei S, Lahmi R, Hanif H, Soleimani M (2015). Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1. Journal of Medical Genetics, 53(1), 24-33.
Chicago	Kouhkan F, Mobarra N, Soufizomorrod M, Keramati F, Ali Hosseini Rad SM, Fathiroudsari M, Tavakoli R, et al.. "Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1." Journal of Medical Genetics 53, no. 1 (2015): 24-33.
Harvard	Kouhkan F et al. (2015) 'Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1', Journal of Medical Genetics, 53(1), pp. 24-33.
Vancouver	Kouhkan F, Mobarra N, Soufizomorrod M, Keramati F, Ali Hosseini Rad SM, Fathiroudsari M, et al.. Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1. Journal of Medical Genetics. 2015;53(1):24-33.
BibTex	@article{ author = {Kouhkan F and Mobarra N and Soufizomorrod M and Keramati F and Ali Hosseini Rad SM and Fathiroudsari M and Tavakoli R and Hajarizadeh A and Ziaei S and Lahmi R and Hanif H and Soleimani M}, title = {Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1}, journal = {Journal of Medical Genetics}, volume = {53}, number = {1}, pages = {24-33}, year = {2015} }
RIS	TY - JOUR AU - Kouhkan F AU - Mobarra N AU - Soufizomorrod M AU - Keramati F AU - Ali Hosseini Rad SM AU - Fathiroudsari M AU - Tavakoli R AU - Hajarizadeh A AU - Ziaei S AU - Lahmi R AU - Hanif H AU - Soleimani M TI - Microrna-129-1 Acts As Tumour Suppressor and Induces Cell Cycle Arrest of Gbm Cancer Cells Through Targeting Igf2bp3 and Mapk1 JO - Journal of Medical Genetics VL - 53 IS - 1 SP - 24 EP - 33 PY - 2015 ER -

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