Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles

Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles Publisher Pubmed

Pournoori N^{1, 2} ; Oghabian MA^{1, 2} ; Irajirad R³ ; Muhammadnejad S^{4, 5} ; Delavari H H⁶

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1. Department of Medical Physics and Biomedical Engineering, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Biomarker Imaging and Analysis Group, Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran
3. Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran
4. Gene Therapy Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Dr. Shariati Hospital, Jalal Al-e Ahmad Hwy, Tehran, Iran
5. Pediatric Cell and Gene Therapy Research Center, Gene, Cell & Tissue Research Institute, Tehran University of Medical Science, Tehran, Iran
6. Department of Materials Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, Iran

Source: ChemMedChem Published:2022

Abstract

Immune checkpoint blockade, considered a revolutionary approach in cancer treatment, is only effective in patients with high tumor-infiltrating lymphocytes (TILs). This work aimed to investigate the feasibility of targeted contrast agent (CA) based on dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs-DEX) for TILs detection by magnetic resonance imaging (MRI) studies. To do so, we synthesized an MRI CA by conjugating SPIONs-DEX to an anti-CD3 monoclonal antibody via cyanogen bromide as a cross-linker. In vitro assessments demonstrated the higher labeling efficiency of the developed CA to CD3+ lymphocytes compared to SPIONs-DEX. In vivo MRI of a xenograft model of CD3+ lymphocytes revealed the significant signal loss after the intravenous injection of the bioconjugate by ∼34 % and 21 % in T2*-weighted and T2-weighted images, respectively. The histopathological evaluation of xenograft tumors confirmed the labeling of lymphocytes by the targeted CA. This approach could open up a new horizon in the non-invasive assessment of TILs to identify patients eligible for immunotherapy. © 2022 Wiley-VCH GmbH.

Style	Citing Format
MLA	Pournoori N, et al.. "Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles." ChemMedChem, vol. 17, no. 11, 2022, pp. -.
APA	Pournoori N, Oghabian MA, Irajirad R, Muhammadnejad S, Delavari H H (2022). Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles. ChemMedChem, 17(11), -.
Chicago	Pournoori N, Oghabian MA, Irajirad R, Muhammadnejad S, Delavari H H. "Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles." ChemMedChem 17, no. 11 (2022): -.
Harvard	Pournoori N et al. (2022) 'Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles', ChemMedChem, 17(11), pp. -.
Vancouver	Pournoori N, Oghabian MA, Irajirad R, Muhammadnejad S, Delavari H H. Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles. ChemMedChem. 2022;17(11):-.
BibTex	@article{ author = {Pournoori N and Oghabian MA and Irajirad R and Muhammadnejad S and Delavari H H}, title = {Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles}, journal = {ChemMedChem}, volume = {17}, number = {11}, pages = {-}, year = {2022} }
RIS	TY - JOUR AU - Pournoori N AU - Oghabian MA AU - Irajirad R AU - Muhammadnejad S AU - Delavari H H TI - Magnetic Resonance Imaging of Tumor-Infiltrating Lymphocytes by Anti-Cd3-Conjugated Iron Oxide Nanoparticles JO - ChemMedChem VL - 17 IS - 11 SP - EP - PY - 2022 ER -

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