Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):

Share this content! On (X network) By

Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells Publisher Pubmed

Nazeri T¹ ; Hedayatpour A² ; Kazemzadeh S² ; Safari M² ; Safi S² ; Khanehzad M³

Show Affiliations

1. Department of Biology, Islamic Azad University, SariBranch, Mazandaran, Iran
2. Department of Anatomy, School of Medicine, Tehran University of Medical Science, Tehran, 1416753955, Iran
3. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 8174673461, Iran

Source: Biopreservation and Biobanking Published:2022

Abstract

Cryopreservation of spermatogonial stem cells (SSCs) is an important method to restore and maintain fertility in preadolescent children suffering from cancer. For protection of SSCs from cryoinjury, various antioxidant agents have been used. The aim of this study was to assess the antiapoptotic and antioxidant effects of melatonin in frozen-thawed SSCs. SSCs were isolated from testes of neonatal mice (3-6 days old) and their purities were measured by flow cytometry with promyelocytic leukemia zinc finger protein. After culturing, the cells were frozen in two groups (1) control and (2) melatonin (100 μM) and stored for 1 month. Finally, the cell viability, colonization rate, expression of Bcl-2 and BAX gene, and intracellular reactive oxygen species (ROS) were evaluated after freezing-thawing. Melatonin increased the viability and colonization of SSCs and Bcl-2 gene expression. It also diminished BAX gene expression and intracellular ROS. The results of this study show that melatonin with antioxidant and antiapoptotic effects can be used as an additive for freezing and long-term storage of cells and infertility treatment in the clinic. © 2022 Mary Ann Liebert, Inc., publishers.

Related Docs

View other Related Docs

1. Effect of a Freezing Medium Containing Melatonin on Markers of Pre-Meiotic and Post-Meiotic Spermatogonial Stem Cells (Sscs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion, Reproductive Sciences (2021)

2. Melatonin in Cryopreservation Media Improves Transplantation Efficiency of Frozen–Thawed Spermatogonial Stem Cells Into Testes of Azoospermic Mice, Stem Cell Research and Therapy (2022)

3. Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model, Reproductive Sciences (2022)

4. Microrna-30A-5P Promotes Differentiation in Neonatal Mouse Spermatogonial Stem Cells (Sscs), Reproductive Biology and Endocrinology (2021)

5. The Effects of Melatonin on Colonization of Neonate Spermatogonial Mouse Stem Cells in a Three-Dimensional Soft Agar Culture System, Stem Cell Research and Therapy (2017)

6. Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel], International Journal of Morphology (2019)

7. Recovering Spermatogenesis by Protected Cryopreservation Using Metformin and Transplanting Spermatogonial Stem Cells Into Testis in an Azoospermia Mouse Model, Biopreservation and Biobanking (2024)

8. Effect of Mir-30A-5P on Apoptosis, Colonization, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells, Biopreservation and Biobanking (2021)

Experts (# of related papers)

View all Related Experts

Mehdi Abbasi (12)

Tayebeh Rastegar (8)

Other Related Docs

9. Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion, Heliyon (2021)

10. Advances in Cryopreservation of Spermatogonial Stem Cells and Restoration of Male Fertility, Microscopy Research and Technique (2016)

11. Tissue Engineering Studies in Male Infertility Disorder, Human Fertility (2023)

12. The Roles of Sertoli Cells in Fate Determinations of Spermatogonial Stem Cells, Tehran University Medical Journal (2016)

13. Melatonin Affects Membrane Integrity, Intracellular Reactive Oxygen Species, Caspase3 Activity and Akt Phosphorylation in Frozen Thawed Human Sperm, Cell and Tissue Research (2018)

14. Astaxanthin Relieves Busulfan-Induced Oxidative Apoptosis in Cultured Human Spermatogonial Stem Cells by Activating the Nrf-2/Ho-1 Pathway, Reproductive Sciences (2022)

15. Pentoxifylline Promotes Spermatogenesis Via Upregulation of the Nrf2-Are Signalling Pathway in a Mouse Model of Germ-Cell Apoptosis Induced by Testicular Torsion-Detorsion, Reproduction# Fertility and Development (2023)

16. Colonization of Neonate Mouse Spermatogonial Stem Cells Co-Culture With Sertoli Cells in the Presence and Absence Soft Agar, Asian Pacific Journal of Reproduction (2020)

17. Protective Effects of Astaxanthin on Post-Thaw Sperm Quality in Normozoospermic Men, Andrologia (2024)

18. Roles for Kisspeptin in Proliferation and Differentiation of Spermatogonial Cells Isolated From Mice Offspring When the Cells Are Cocultured With Somatic Cells, Journal of Cellular Biochemistry (2019)

19. A Systematic Review on the Role of Melatonin and Its Mechanisms on Diabetes-Related Reproductive Impairment in Non-Clinical Studies, Frontiers in Endocrinology (2022)

20. The Role of Melatonin on Chemotherapy-Induced Reproductive Toxicity, Journal of Pharmacy and Pharmacology (2018)

21. In Vitro Effects of Melatonin on Colonization of Neonate Mouse Spermatogonial Stem Cells, Systems Biology in Reproductive Medicine (2017)

22. Human Menstrual Blood Stem Cell-Derived Granulosa Cells Participate in Ovarian Follicle Formation in a Rat Model of Premature Ovarian Failure in Vivo, Cellular Reprogramming (2019)

23. Antioxidant Effects of Quercetin in Freeze-Thawing Process of Mouse Spermatogonial Stem Cells, Asian Pacific Journal of Reproduction (2019)

24. In Vitro Spermatogenesis in Artificial Testis: Current Knowledge and Clinical Implications for Male Infertility, Cell and Tissue Research (2023)

25. Improving the Efficacy of Cryopreservation of Spermatogonia Stem Cells by Antioxidant Supplements, Cellular Reprogramming (2016)

26. Insights Into the Role of Melatonin on the Reproductive System Toxicity Induced by Bisphenol A: A Systematic Review of Preclinical Studies, Toxin Reviews (2024)

27. Differentiation of Spermatogonial Stem Cells by Soft Agar Three-Dimensional Culture System, Artificial Cells# Nanomedicine and Biotechnology (2019)

28. Three-Dimensional Co-Culture of Human Spermatogonial Stem Cells With Sertoli Cells in Soft Agar Culture System Supplemented by Growth Factors and Laminin, Acta Histochemica (2020)

29. Advances of Three-Dimensional (3D) Culture Systems for in Vitro Spermatogenesis, Stem Cell Research and Therapy (2023)

30. Efficiency of Colony Formation and Differentiation of Human Spermatogenic Cells in Two Different Culture Systems, Reproductive Biology (2018)

31. Protective Effects of the Antioxidant Capacity of Curcumin Against Apoptosis on the Expression Level of Apoptosis-Related Caspase-3 Gene in Varicocelized Rats, Surgical Chronicles (2021)

32. Alteration of Spermatogenesis Following Spermatogonial Stem Cells Transplantation in Testicular Torsion-Detorsion Mice, Journal of Assisted Reproduction and Genetics (2016)

Style	Citing Format
MLA	Nazeri T, et al.. "Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells." Biopreservation and Biobanking, vol. 20, no. 4, 2022, pp. 374-383.
APA	Nazeri T, Hedayatpour A, Kazemzadeh S, Safari M, Safi S, Khanehzad M (2022). Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells. Biopreservation and Biobanking, 20(4), 374-383.
Chicago	Nazeri T, Hedayatpour A, Kazemzadeh S, Safari M, Safi S, Khanehzad M. "Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells." Biopreservation and Biobanking 20, no. 4 (2022): 374-383.
Harvard	Nazeri T et al. (2022) 'Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells', Biopreservation and Biobanking, 20(4), pp. 374-383.
Vancouver	Nazeri T, Hedayatpour A, Kazemzadeh S, Safari M, Safi S, Khanehzad M. Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells. Biopreservation and Biobanking. 2022;20(4):374-383.
BibTex	@article{ author = {Nazeri T and Hedayatpour A and Kazemzadeh S and Safari M and Safi S and Khanehzad M}, title = {Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells}, journal = {Biopreservation and Biobanking}, volume = {20}, number = {4}, pages = {374-383}, year = {2022} }
RIS	TY - JOUR AU - Nazeri T AU - Hedayatpour A AU - Kazemzadeh S AU - Safari M AU - Safi S AU - Khanehzad M TI - Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells JO - Biopreservation and Biobanking VL - 20 IS - 4 SP - 374 EP - 383 PY - 2022 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract