Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk

Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk Publisher Pubmed

Besharati M^{1, 2} ; Tabrizi MA³ ; Molaabasi F⁴ ; Saber R⁵ ; Shamsipur M⁶ ; Hamedi J^{1, 2} ; Hosseinkhani S⁷

Show Affiliations

1. Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran
2. Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran
3. Electronic Technology Department, Universidad Carlos III de Madrid, Madrid, Getafe, Spain
4. Department of Biomaterials and Tissue Engineering, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
5. Research Center of Medical Science, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Chemistry, Razi University, Kermanshah, Iran
7. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Source: Biotechnology and Applied Biochemistry Published:2022

Abstract

Recently, there has been a growing demand to develop portable devices for the fast detection of contaminants in food safety, healthcare, and environmental fields. Herein, two biosensing methods were designed by the use of nicotinamide adenine dinucleotide phosphate (NAD(P)H)-dependent TetX2 enzyme activity and thionine as an excellent electrochemical and colorimetric mediator/probe to monitor tetracycline (TC) in milk. The nanoporous glassy carbon electrode (NPGCE) modified with polythionine was first prepared by electrochemically and then TetX2 was immobilized onto the NPGCE using polyethyleneimine. The prepared biosensor provided a high electrocatalytic response toward NAD(P)H by significantly reducing its overpotential. The proposed biosensor exhibited a detection limit of 40 nM with a linear range of 0.1–0.8 μM for TC determination. Besides, the thionine probe was used to develop a novel colorimetric assay using a simple enzymatic color reaction within a few minutes. The limit of detection for TC was experimentally achieved as 60 nM, which was lower than the safety levels established by the World Health Organization (225 nM). The correlation between change in the color of the solution and the concentration of TC was used for quality control of milk samples, as confirmed by the standard high-performance liquid chromatography method. The results show the great potential of the proposed assays as portable instruments for on-site TC measurements. © 2020 International Union of Biochemistry and Molecular Biology, Inc.

Style	Citing Format
MLA	Besharati M, et al.. "Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk." Biotechnology and Applied Biochemistry, vol. 69, no. 1, 2022, pp. 41-50.
APA	Besharati M, Tabrizi MA, Molaabasi F, Saber R, Shamsipur M, Hamedi J, Hosseinkhani S (2022). Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk. Biotechnology and Applied Biochemistry, 69(1), 41-50.
Chicago	Besharati M, Tabrizi MA, Molaabasi F, Saber R, Shamsipur M, Hamedi J, Hosseinkhani S. "Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk." Biotechnology and Applied Biochemistry 69, no. 1 (2022): 41-50.
Harvard	Besharati M et al. (2022) 'Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk', Biotechnology and Applied Biochemistry, 69(1), pp. 41-50.
Vancouver	Besharati M, Tabrizi MA, Molaabasi F, Saber R, Shamsipur M, Hamedi J, et al.. Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk. Biotechnology and Applied Biochemistry. 2022;69(1):41-50.
BibTex	@article{ author = {Besharati M and Tabrizi MA and Molaabasi F and Saber R and Shamsipur M and Hamedi J and Hosseinkhani S}, title = {Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk}, journal = {Biotechnology and Applied Biochemistry}, volume = {69}, number = {1}, pages = {41-50}, year = {2022} }
RIS	TY - JOUR AU - Besharati M AU - Tabrizi MA AU - Molaabasi F AU - Saber R AU - Shamsipur M AU - Hamedi J AU - Hosseinkhani S TI - Novel Enzyme-Based Electrochemical and Colorimetric Biosensors for Tetracycline Monitoring in Milk JO - Biotechnology and Applied Biochemistry VL - 69 IS - 1 SP - 41 EP - 50 PY - 2022 ER -

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