Sensitive Detection of H2o2 Released From Cancer Cells With Electrochemiluminescence Sensor Based on Electrochemically Prepared Polypyrrole@Ce: Dy Tungstate/Polyluminol Publisher



Sobhanie E¹ ; Hosseini M^{2, 3} ; Faridbod F¹ ; Ganjali MR¹ Show Affiliations
Source: Journal of Electroanalytical Chemistry Published:2023

Abstract

The sensitive and rapid detection of H2O2 release from living cells has essential significance in the early diagnosis of many diseases. In this manuscript, a solid-state electrochemiluminescence (ECL) sensor was fabricated for ultrasensitive detection of Hydrogen peroxide (H2O2) with a comprehensive linear response in the ranges of 1 pmol/L to 10 μmol/L and a low detection limit of 0.198 pmol/L limit of quantification is 0.602 pmol/L. Briefly, The ECL sensor was developed based on electrodeposition layers of polypyrrole-Ce: Dy(WO4)3 nanocomposite for surface modification. Then poly Luminol was immobilized by electropolymerization on a glassy carbon electrode (GCE) surface as an ECL emitter. The properties of polypyrrole-Ce: Dy(WO4)3 nanocomposite and the modified electrode were evaluated using FE-SEM, Cyclic voltammetry (CV), Electrochemical impedance spectroscopy (EIS), and Atomic force microscopy (AFM) methods. Moreover, the ECL response of the PPy-Ce: Dy(WO4)3/Polyluminol electrode was applied to monitor the H2O2 level release from breast cancer cells (MCF-7). The ECL intensity was related to the logarithm of MCF-7 cell numbers, and the detection limit was 30 cells mol/L. Consequently, this ECL sensor was successfully applied to detect H2O2 released from living cancer cells with the advantages of sensitivity, reproducibility, rapid response, long-term stability, ease of operation, and low cost. © 2023 Elsevier B.V.