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Cdte Amplification Nanoplatforms Capped With Thioglycolic Acid for Electrochemical Aptasensing of Ultra-Traces of Atp Publisher Pubmed



Shamsipur M1 ; Farzin L2 ; Tabrizi MA3 ; Shanehsaz M4
Authors
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Authors Affiliations
  1. 1. Department of Chemistry, Razi University, P.O. Box 67149-67346, Kermanshah, Iran
  2. 2. School of Chemistry, College of Science, University of Tehran, P.O. Box 14174-66191, Tehran, Iran
  3. 3. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, P.O. Box 14197-33131, Tehran, Iran
  4. 4. Analytical Chemistry Research Laboratory, Mobin Shimi Azma Company, P.O. Box 14768-44949, Tehran, Iran

Source: Materials Science and Engineering C Published:2016


Abstract

A “signal off” voltammetric aptasensor was developed for the sensitive and selective detection of ultra-low levels of adenosine triphosphate (ATP). For this purpose, a new strategy based on the principle of recognition-induced switching of aptamers from DNA/DNA duplex to DNA/target complex was designed using thioglycolic acid (TGA)-capped CdTe quantum dots (QDs) as the signal amplifying nano-platforms. Owing to the small size, high surface-to-volume ratio and good conductivity, quantum dots were immobilized on the electrode surface for signal amplification. In this work, methylene blue (MB) adsorbed to DNA was used as a sensitive redox reporter. The intensity of voltammetric signal of MB was found to decrease linearly upon ATP addition over a concentration range of 0.1 nM to 1.6 μM with a correlation coefficient of 0.9924. Under optimized conditions, the aptasensor was able to selectively detect ATP with a limit of detection of 45 pM at 3σ. The results also demonstrated that the QDs-based amplification strategy could be feasible for ATP assay and presented a potential universal method for other small biomolecular aptasensors. © 2016 Elsevier B.V.
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