Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization

Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization Publisher Pubmed

Salehinik N^{1, 3, 4} ; Malaiebalasi Z² ; Amoabediny G^{1, 2} ; Banikarimi SP^{1, 2} ; Zandiehdoulabi B^{3, 4} ; Kleinnulend J^{3, 4}

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1. School of Chemical Engineering, College of Engineering, University of Tehran, 111554563, Enqelab Avenue, Tehran, Iran
2. Research Center for New Technologies in Life Science Engineering, University of Tehran, 143951374, Enqelab Avenue, Tehran, Iran
3. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3008, Amsterdam, 1081, LA, Netherlands
4. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, Velenjak, Shahid Chamran Highway, Tehran, Iran

Source: Materials Science and Engineering C Published:2017

Abstract

Biocompatibility of biomedical devices can be improved by endothelialization of blood-contacting parts mimicking the vascular endothelium's function. Improved endothelialization might be obtained by using biomimetic coatings that allow local sustained release of biologically active molecules, e.g. anti-thrombotic and growth-inducing agents, from nanoliposomes. We aimed to test whether incorporation of growth-inducing nanoliposomal growth hormone (nGH) and anti-thrombotic nanoliposomal sodium nitrite (nNitrite) into collagen coating of silicone tubes enhances endothelialization by stimulating endothelial cell proliferation and inhibiting platelet adhesion. Collagen coating stably immobilized on acrylic acid-grafted silicone tubes decreased the water contact angle from 102° to 56°. Incorporation of 50 or 500 nmol/ml nNitrite and 100 or 1000 ng/ml nGH into collagen coating decreased the water contact angle further to 48°. After 120 h incubation, 58% nitrite and 22% GH of the initial amount of sodium nitrite and GH in nanoliposomes were gradually released from the nNitrite-nGH-collagen coating. Endothelial cell number was increased after surface coating of silicone tubes with collagen by 1.6-fold, and with nNitrite-nGH-collagen conjugate by 1.8–3.9-fold after 2 days. After 6 days, endothelial cell confluency in the absence of surface coating was 22%, with collagen coating 74%, and with nNitrite-nGH-collagen conjugate coating 83–119%. In the absence of endothelial cells, platelet adhesion was stimulated after collagen coating by 1.3-fold, but inhibited after nNitrite-nGH-collagen conjugate coating by 1.6–3.7-fold. The release of anti-thrombotic prostaglandin I2 from endothelial cells was stimulated after nNitrite-nGH-collagen conjugate coating by 1.7–2.2-fold compared with collagen coating. Our data shows improved endothelialization and blood compatibility using nNitrite-nGH-collagen conjugate coating on silicone tubes suggesting that these coatings are highly suitable for use in blood-contacting parts of biomedical devices. © 2017

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Style	Citing Format
MLA	Salehinik N, et al.. "Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization." Materials Science and Engineering C, vol. 77, no. , 2017, pp. 1204-1215.
APA	Salehinik N, Malaiebalasi Z, Amoabediny G, Banikarimi SP, Zandiehdoulabi B, Kleinnulend J (2017). Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization. Materials Science and Engineering C, 77(), 1204-1215.
Chicago	Salehinik N, Malaiebalasi Z, Amoabediny G, Banikarimi SP, Zandiehdoulabi B, Kleinnulend J. "Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization." Materials Science and Engineering C 77, no. (2017): 1204-1215.
Harvard	Salehinik N et al. (2017) 'Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization', Materials Science and Engineering C, 77(), pp. 1204-1215.
Vancouver	Salehinik N, Malaiebalasi Z, Amoabediny G, Banikarimi SP, Zandiehdoulabi B, Kleinnulend J. Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization. Materials Science and Engineering C. 2017;77():1204-1215.
BibTex	@article{ author = {Salehinik N and Malaiebalasi Z and Amoabediny G and Banikarimi SP and Zandiehdoulabi B and Kleinnulend J}, title = {Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization}, journal = {Materials Science and Engineering C}, volume = {77}, number = {}, pages = {1204-1215}, year = {2017} }
RIS	TY - JOUR AU - Salehinik N AU - Malaiebalasi Z AU - Amoabediny G AU - Banikarimi SP AU - Zandiehdoulabi B AU - Kleinnulend J TI - Sustained Release of Growth Hormone and Sodium Nitrite From Biomimetic Collagen Coating Immobilized on Silicone Tubes Improves Endothelialization JO - Materials Science and Engineering C VL - 77 IS - SP - 1204 EP - 1215 PY - 2017 ER -

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