Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma

Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma

Bahrami N^{1, 2} ; Gholami M³ ; Jamaati HR⁴ ; Mohamadnia A^{5, 6} ; Dargahi H⁷ ; Kazempour Dizaji M⁸ ; Khosravi A⁹ ; Heshmat Nia J¹⁰ ; Vahabi P¹¹ ; Bahrami N^{1, 2}

Show Affiliations

1. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4. Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Virology Research Center, National Research Institute of Tuberculosis and Lung, Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. Biotechnology Department, Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Allied Medicine, Health Information Management Research Center, Tehran University Of Medical Sciences, Tehran, Iran
8. Biostatistics Department, Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Teheran, Iran
9. Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
10. National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
11. School of Nursing, Tehran University of Medical Sciences, Tehran, Iran
12. Department of Biomedical Engineering, Virginia Tech-Wake Forest University, Blacksburg, VA, United States
13. Wake Forest University, School of Medicine, Winston-Salem, NC, United States

Source: Minerva Pneumologica Published:2016

Abstract

BACKGROUND: Despite medical advances, lung cancer often remains undiagnosed until advanced stages mainly due to the lack of specific tumor markers. This study sought to assess vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) mRNA biomarkers in the peripheral blood of non-small cell lung carcinoma (NSCLC) patients for possible use in diagnosis and early screening of these patients. METHODS: Peripheral blood samples were drawn of 30 NSCLC patients and 30 healthy controls; RNA was extracted and of each RNA sample, cDNA was synthesized in triplicate. Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was used to quantify VEGF and CEA mRNA biomarkers. RESULTS: Of all patients, 86.6% (26 out of 30) were positive for VEGF mRNA; this rate was 13.3% (4 out of 30) in healthy controls. Of all patients, 90% (27 out of 30) were positive for CEA mRNA; this rate was 33.3% (10 out of 30) in healthy controls. CONCLUSIONS: Based on the results, VEGF and CEA mRNA seem to be beneficial biomarkers for the diagnosis of NSCLC using peripheral blood samples. However, simultaneous use of several molecular biomarkers is proposed to achieve more reliable results. © 2016 EDIZIONI MINERVA MEDICA.

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Naghmeh Bahrami (8)

Seyed Hamidollah Ghaffari (1)

Style	Citing Format
MLA	Bahrami N, et al.. "Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma." Minerva Pneumologica, vol. 55, no. 3, 2016, pp. 31-36.
APA	Bahrami N, Gholami M, Jamaati HR, Mohamadnia A, Dargahi H, Kazempour Dizaji M, Khosravi A, Heshmat Nia J, Vahabi P, Bahrami N (2016). Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma. Minerva Pneumologica, 55(3), 31-36.
Chicago	Bahrami N, Gholami M, Jamaati HR, Mohamadnia A, Dargahi H, Kazempour Dizaji M, Khosravi A, Heshmat Nia J, Vahabi P, Bahrami N. "Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma." Minerva Pneumologica 55, no. 3 (2016): 31-36.
Harvard	Bahrami N et al. (2016) 'Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma', Minerva Pneumologica, 55(3), pp. 31-36.
Vancouver	Bahrami N, Gholami M, Jamaati HR, Mohamadnia A, Dargahi H, Kazempour Dizaji M, et al.. Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma. Minerva Pneumologica. 2016;55(3):31-36.
BibTex	@article{ author = {Bahrami N and Gholami M and Jamaati HR and Mohamadnia A and Dargahi H and Kazempour Dizaji M and Khosravi A and Heshmat Nia J and Vahabi P and Bahrami N}, title = {Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma}, journal = {Minerva Pneumologica}, volume = {55}, number = {3}, pages = {31-36}, year = {2016} }
RIS	TY - JOUR AU - Bahrami N AU - Gholami M AU - Jamaati HR AU - Mohamadnia A AU - Dargahi H AU - Kazempour Dizaji M AU - Khosravi A AU - Heshmat Nia J AU - Vahabi P AU - Bahrami N TI - Expression of Two Essential Mrna Biomarker in the Peripheral Blood As Possible Biomarkers for Diagnosis of Non-Small Cell Lung Carcinoma JO - Minerva Pneumologica VL - 55 IS - 3 SP - 31 EP - 36 PY - 2016 ER -

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