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Detection of Vascular Endothelial Growth Factor Messenger Rna (Vegf Mrna), Cytokeratin-19 Mrna (Ck19 Mrna), and Vascular Endothelial Growth Factor (Vegf) Protein Biomarker in the Peripheral Blood of Patients With Breast Cancer



Khebreh H1 ; Mohamadnia A2 ; Falahati M1 ; Bahrami N3
Authors
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Authors Affiliations
  1. 1. Department of Molecular and Cellular Sciences, School of Advance Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  2. 2. Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital AND Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Craniomaxillofacial Research center and Iranian Tissue Bank Research Center, Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Isfahan Medical School Published:2017

Abstract

Background: Breast cancer is a multifactorial potentially lethal disease that is triggered by genetic factors as well as numerous environmental factors. The present research aimed to examine the expression of Vascular endothelial growth factor messenger RNA (VEGF mRNA), cytokeratin-19 mRNA (CK19 mRNA), and vascular endothelial growth factor (VEGF) protein biomarker in the peripheral blood of patients with breast cancer. Methods: 40 patients with breast cancer were compared to 40 healthy individuals. The real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) method was used to determine the expressions of the CK19 mRNA and VEGF mRNA biomarkers in the peripheral blood samples of the healthy participants and the patients. The VEGF protein was also compared using the (ELISA) method. Findings: The positive VEGF mRNA biomarker was observed in 30 of the 40 patients with breast cancer; thus the sensitivity of this marker was 75%. In the healthy participants group, 6 of the 40 participants showed a positive VEGF mRNA biomarker expression. The CK19 mRNA marker was positive in 25 of the 40 patients, which indicated a sensitivity of 62.5%. In the healthy participants group, the positive expression of the CK19 mRNA biomarker was observed in 7 of the 40 participants. VEGF was positive in 27 of the 40 patients. In the control group, 5 of the 40 participants showed the positive expression of this biomarker. Conclusion: In sum, based on the results of this research, the assessed breast cancer tumor markers can be used as screening tests for the early diagnosis of patients. To further prove the findings of this study, more extensive studies with larger sample sizes are recommended. © 2017, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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