Efect of Met-Anandamide on Prevention of Hyperactivation, Cryo-Capacitation and Acrosome Reaction in Ram Semen Cryopreservation; [Met-Anandamidin Koc Spermasinin Dondurulmasinda Hiperaktivasyon, Kryo-Kapasitasyon Ve Akrozom Reaksiyonunun Onlenmesi Uzerine Etkisi] Publisher



Talebiyan R¹ ; Amidi F² ; Samini M³ ; Mirshokraei P^{4, 5} ; Dehkordi SH⁶ Show Affiliations
Source: Kafkas Universitesi Veteriner Fakultesi Dergisi Published:2015

Abstract

Cryopreserved spermatozoon has some defects on membrane causing hyper activation, cryo-capacitation and timeless (early) acrosome reaction that lead to low fertility. While low fertility is generally accepted as a consequence of cryopreservation of sperm cells, a lot of studies are being carried out on cryopreservation techniques to improve spermatozoon membrane integrity and also viability. Some studies point out that using materials such as Cannabinoids can be efective in achieving this aim. The main efects of endocannabinoids on spermatozoa are the inhibition of motility, capacitation and acrosome reaction. The aim of this study was to determine the efect of soluted Met-anandamide in extender on post thawing sperm motility, capacitation and acrosome reaction in ram semen cryopreservation. For this aim, ten ejaculated collected from five mature Bakhtiari rams were diluted with extender containing Met-anandamide, equilibrated and then cryopreserved in liquid nitrogen vapour (approximately -125 to -130°C). Cryopreserved semen samples were preserved in liquid nitrogen until evaluation. Post thawing sperm analyses revealed that the Cannabinoids agonists afected the motility of ram sperm after thawing (P<0.05), resulting in an increase in the AEA (arachidonoylethanolamide) concentration (10, 100 and 1000 nM) which significantly reduced sperm motility. subsequent freezing and thawing. On the other hand, the degrading enzyme of endocannabinoid FAAH may have had a reduction efect on sperm motility and increased capacitation and early acrosome reaction (P<0.05). In conclusion, endocannabinoid system may be used to improve ram sperm freezing. © 2015, Veteriner Fakultesi Dergisi. All rights reserved.