Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Cryopreservation and Its Effects on Motility and Gene Expression Patterns and Fertilizing Potential of Bovine Epididymal Sperm Publisher Pubmed



Nazari H1 ; Ahmadi E1 ; Hosseini Fahraji H2 ; Afzali A3 ; Davoodian N1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
  2. 2. PhD Student of Animal Reproductive Biotechnology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
  3. 3. PhD Candidate of Reproductive Biology, Faculty of Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

Source: Veterinary Medicine and Science Published:2021


Abstract

Despite encountering new challenges in using epididymal sperm recovered from cauda epididymides, this accessible and, in some species, worthwhile sample makes inevitable the further development of a suitable cryopreservation protocol. In this study, sperm was recovered from the epididymis of 4°C overnight stored slaughtered bulls' testes and the effects of cryopreservation on the bovine epididymal sperm motility (with CASA) and gene expression patterns (with quantitative Real time-PCR) were evaluated. Moreover the fertilizing potential of cryopreserved epididymal sperm was used in in vitro fertilization (IVF). After freezing and thawing of epididymal sperm, total and slow progressive sperm motility, VCL, VAP, MAD, ALH and BCF were significantly decreased (p <.05), while in the parameters of fast progressive motility, VSL, LIN, WOB and STR there were not any significant variations in the frozen sperm compared to fresh (non-frozen) counterpart. The assessment of abundance of transcripts encoding motility (TSSK6) and fertility (PRM1 and PRM2)-related genes in epididymal sperm, showed that these transcripts were affected by freezing especially in slow progressive motility status (p <.01). Furthermore, cleavage and blastocyst rate did not present any significant differences between bovine embryos produced in vitro by fresh or frozen-thawed epididymal sperm. It can be concluded that epididymal sperm has enough freezability after overnight testes storage, and cryopreservation could not affect the percentage of in vitro produced embryos in spite of the changes of relative abundance of some transcripts and direction progressive motility pattern of sperm. © 2020 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd
Related Docs
1. Prospect and Competence of Quantitative Methods Via Real-Time Pcr in a Comparative Manner: An Experimental Review of Current Methods, Open Bioinformatics Journal (2018)
2. The Effect of Cryopreservation on Dna Methylation Patterns of the Chromosome 15Q11–Q13 Region in Human Spermatozoa, Cell and Tissue Banking (2020)
3. Effect of Sulforaphane on Apoptosis, Reactive Oxygen Species and Lipids Peroxidation of Human Sperm During Cryopreservation, Cryobiology (2021)
4. The Relationship Between Preeclampsia Risk and Sencr Rs555172 Gene Polymorphism and Expression, Cellular and Molecular Biology (2024)
5. Dysregulation of Angiogenesis and Inflammatory Genes in Endometrial Mesenchymal Stem Cells and Their Contribution to Endometriosis, Iranian Journal of Allergy# Asthma and Immunology (2021)
6. Protective Effects of Hesperetin on the Quality of Sperm, Apoptosis, Lipid Peroxidation, and Oxidative Stress During the Process of Cryopreservation: An Experimental Study, International Journal of Reproductive BioMedicine (2021)
7. Effect of Trolox on Sperm Quality in Normozospermia and Oligozospermia During Cryopreservation, Cryobiology (2016)
Experts (# of related papers)
View all Related Experts
Fardin Amidi (2)
Maryam Shabani Nashtaee (2)