Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro

Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro Publisher Pubmed

Nikoomanesh F¹ ; Roudbarmohammadi S¹ ; Khoobi M² ; Haghighi F³ ; Roudbary M⁴

Show Affiliations

1. Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3. Microbiology, School of Dentistry, University of California, Los Angeles, CA, United States
4. Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2019

Abstract

The evolution of drug resistance of Candida species to conventional antifungal agents has been a major medical challenge worldwide; attempt to use the potential antifungal agents with appropriate therapy efficacy and minimum effects is considerably growing. This study was conducted to evaluate the use of nanogel as a nanocarrier for pharmaceutical application of farnesol. The nanogels were synthetized using alginate (AL) and chitosan (CS) polymers containing 300 µM of farnesol in the nano-range 42–70 nm size. In vitro release studies indicated that release of farnesol from CS and AL nanogels was as 58% and 37%, respectively. Chitosan nanogel showed more in inhibitory zone as compared to AL nanogel (9 mm). Also, cytotoxicity assay showed no significant difference between control and treatment groups (p>.05). Finally, the effect of nanogels on genes expression of HWP1, SAP6 and Rim101 in Candida albicans ATCC10231 was assessed using real-time polymerase chain reaction (PCR). Expression of HWP1 and SAP6 genes in C. albicans treated with CS nanogel was significantly decreased (p<.01). In general, the obtained finding showed that, CS nanogel contains farnesol with proper antifungal activity and as a new approach used in pharmaceutical applications against C. albicans; however, more studies in vitro and in vivo are needed in the future. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

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Style	Citing Format
MLA	Nikoomanesh F, et al.. "Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro." Artificial Cells# Nanomedicine and Biotechnology, vol. 47, no. 1, 2019, pp. 64-72.
APA	Nikoomanesh F, Roudbarmohammadi S, Khoobi M, Haghighi F, Roudbary M (2019). Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro. Artificial Cells# Nanomedicine and Biotechnology, 47(1), 64-72.
Chicago	Nikoomanesh F, Roudbarmohammadi S, Khoobi M, Haghighi F, Roudbary M. "Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro." Artificial Cells# Nanomedicine and Biotechnology 47, no. 1 (2019): 64-72.
Harvard	Nikoomanesh F et al. (2019) 'Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro', Artificial Cells# Nanomedicine and Biotechnology, 47(1), pp. 64-72.
Vancouver	Nikoomanesh F, Roudbarmohammadi S, Khoobi M, Haghighi F, Roudbary M. Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro. Artificial Cells# Nanomedicine and Biotechnology. 2019;47(1):64-72.
BibTex	@article{ author = {Nikoomanesh F and Roudbarmohammadi S and Khoobi M and Haghighi F and Roudbary M}, title = {Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro}, journal = {Artificial Cells# Nanomedicine and Biotechnology}, volume = {47}, number = {1}, pages = {64-72}, year = {2019} }
RIS	TY - JOUR AU - Nikoomanesh F AU - Roudbarmohammadi S AU - Khoobi M AU - Haghighi F AU - Roudbary M TI - Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro JO - Artificial Cells# Nanomedicine and Biotechnology VL - 47 IS - 1 SP - 64 EP - 72 PY - 2019 ER -

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