Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines

Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines Publisher Pubmed

Atri S¹ ; Nasoohi N¹ ; Hodjat M²

Show Affiliations

1. Department of Biochemistry, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Current Molecular Pharmacology Published:2021

Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) is one of the most fatal malignancies worldwide and despite using various therapeutic strategies for the treatment of HNSCC, the surveillance rate is low. Telomerase has been remarked as the primary target in cancer therapy. Considering the key regulatory role of epigenetic mechanisms in controlling genome expression, the present study aimed to investigate the effects of two epigenetic modulators, a DNA methylation inhibitor and a histone deacetylase inhibitor on cell migration, proliferation, hTERT gene expression, and telomerase activity in HNSCC cell lines. Methods: Human HNSCC cell lines were treated with Azacitidine and Trichostatin A to investigate their effects on telomerase gene expression and activity. Cell viability, migration, hTERT gene expression, and telomerase activity were studied using MTT colorimetric assay, scratch wound assay, qRT-PCR, and TRAP assay, respectively. Results: Azacitidine at concentrations of ≤1μM and Trichostatin A at 0.1 to 0.3nM concentrations significantly decreased FaDu and Cal-27 cells migration. The results showed that Azacitidine significantly decreased hTERT gene expression and telomerase activity in FaDu and Cal-27 cell lines. However, there were no significant changes in hTERT gene expression at different concentrations of Trichostatin A in both cell lines. Trichostatin A treatment affected telomerase activity at the high dose of 0.3 nM Trichostatin A. Conclusion: The findings revealed that unlike histone deacetylase inhibitor, Azacitidine as an inhibitor of DNA methylation decreases telomerase expression in HNSCC cells. This might suggest the potential role of DNA methyltransferase inhibitors in telomerase-based therapeutic approaches in squamous cell carcinoma. © 2021 Bentham Science Publishers.

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Experts (# of related papers)

Mahshid Hodjat (1)

Style	Citing Format
MLA	Atri S, Nasoohi N, Hodjat M. "Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines." Current Molecular Pharmacology, vol. 14, no. 1, 2021, pp. 60-67.
APA	Atri S, Nasoohi N, Hodjat M (2021). Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines. Current Molecular Pharmacology, 14(1), 60-67.
Chicago	Atri S, Nasoohi N, Hodjat M. "Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines." Current Molecular Pharmacology 14, no. 1 (2021): 60-67.
Harvard	Atri S, Nasoohi N, Hodjat M (2021) 'Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines', Current Molecular Pharmacology, 14(1), pp. 60-67.
Vancouver	Atri S, Nasoohi N, Hodjat M. Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines. Current Molecular Pharmacology. 2021;14(1):60-67.
BibTex	@article{ author = {Atri S and Nasoohi N and Hodjat M}, title = {Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines}, journal = {Current Molecular Pharmacology}, volume = {14}, number = {1}, pages = {60-67}, year = {2021} }
RIS	TY - JOUR AU - Atri S AU - Nasoohi N AU - Hodjat M TI - Azacitidine, As a Dnmt Inhibitor Decreases Htert Gene Expression and Telomerase Activity More Effective Compared With Hdac Inhibitor in Human Head and Neck Squamous Cell Carcinoma Cell Lines JO - Current Molecular Pharmacology VL - 14 IS - 1 SP - 60 EP - 67 PY - 2021 ER -

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