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Preparation, Characterization and in Vitro Evaluation of Insulin-Phbv Nanoparticles/Alginate Hydrogel Composite System for Prolonged Delivery of Insulin Publisher Pubmed



Bayrami S1 ; Chamani M1 ; Jamalimoghadamsiahkali S2 ; Seyedalinaghi S3 ; Shirmard LR4 ; Bayrami S1 ; Javar HA1 ; Ghahremani MH6 ; Amini M7, 8 ; Tehrani MR1 ; Shahsavari S9 ; Dorkoosh FA1, 10
Authors
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Authors Affiliations
  1. 1. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Ziaeian Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Iranian Research Center for HIV/AIDS, Iranian Institute for Reduction of High Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmaceutics, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran
  5. 5. Islamic Azad University, North Tehran Branch, Faculty of Bioscience, Tehran, Iran
  6. 6. Department of Toxicology-Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Chemical Engineering Department, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
  10. 10. Medical Biomaterial Research Centre (MBRC), Tehran University of Medical Sciences, Tehran, 14399-56131, Iran

Source: Journal of Pharmaceutical Sciences Published:2024


Abstract

Purpose: In the present study, biodegradable poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles (NPs) containing insulin were loaded in sodium alginate/jeffamine (ALG/jeff) hydrogel for prolonged delivery of insulin. The main aim of this work was to fabricate an efficient insulin delivery system to improve patient adherence by decreasing the repetition of injections. Methods: Swelling and morphological properties and crosslinking efficiency of ALG/jeff hydrogel were assessed. The composite hydrogel was prepared by adding PHBV NPs to ALG/jeff hydrogel concurrently with crosslinking process. The morphology and loading capacity of composite hydrogel were analyzed. Results: Circular dichroism measurement demonstrated that insulin remains stable following fabrication process. The release profile exhibited 54.6 % insulin release from composite hydrogel within 31 days with minor initial burst release equated to nanoparticles and hydrogels. MTT cell viability analysis was performed by applying L-929 cell line and no cytotoxic effect was observed. Conclusions: Favorable results clearly introduced fabricated composite hydrogel as an excellent candidate for drug delivery systems and also paves the route for prolonged delivery systems of other proteins. © 2024
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