The Potential of the Mineralized Bone Allograft Block As an Appropriate Candidate for Bone Tissue Engineering in Periodontology Publisher



Tabatabaee S¹ ; Delyanee M² ; Samanipour R³ ; Tavakoli A⁴ Show Affiliations
Source: Journal of Materials Research Published:2023

Abstract

In this research, an allograft mineralized bone block was processed through decellularizing and lyophilizing after approving the sterility evaluations of the donor. According to SEM images, the block was an interconnected porous structure with pore sizes ranging between 124 and 930 µm which comprised various sizes desirable for bone regeneration. Also, emergence of hydroxyapatite crystals on the scaffold after immersing in simulated body fluid indicated its bioactivity. Furthermore, its mechanical strength was 33.10 ± 0.34 MPa which is suitable for a bone scaffold as load-bearing tissue. The successful decellularization was approved by hematoxylin–eosin staining assay and the scaffold exhibited no cytotoxicity utilizing MTT test. Furthermore, the obtained SEM images after cell seeding as well as the DAPI staining results demonstrated the supporting of cell attachment and proliferation by the scaffold. Therefore, it could be concluded that the prepared allograft mineralized bone block is an appropriate candidate for bone tissue regeneration in periodontology. Graphical abstract: The process of mineralized bone allograft block production and characterization.[Figure not available: see fulltext.]. © 2023, The Author(s), under exclusive licence to The Materials Research Society.