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Differentiation of Neural Crest Stem Cells From Nasal Mucosa Into Motor Neuron-Like Cells Publisher Pubmed



Bagher Z1 ; Kamrava SK1 ; Alizadeh R1 ; Farhadi M1 ; Absalan M2 ; Falah M1 ; Faghihi F3 ; Zaresadeghi A4 ; Komeili A5
Authors
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Authors Affiliations
  1. 1. ENT and Head & Neck Research Center and Department, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences (IUMS), Tehran, Iran
  2. 2. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Cellular and molecular research center, Iran university of Medical Sciences, Tehran, Iran
  4. 4. Skull Base Research Center, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences (IUMS), Tehran, Iran
  5. 5. Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

Source: Journal of Chemical Neuroanatomy Published:2018


Abstract

Cell transplantation is a potential therapeutic approach for repairing neuropathological and neurodegenerative disorders of central nervous system by replacing the degenerated cells with new ones. Among a variety of stem cell candidates to provide these new cells, olfactory ectomesenchymal stem cells (OE-MSCs) have attracted a great attention due to their neural crest origin, easy harvest, high proliferation, and autologous transplantation. Since there is no report on differentiation potential of these cells into motor neuron-like cells, we evaluated this potential using Real-time PCR, flowcytometry and immunocytochemistry after the treatment with differentiation cocktail containing retinoic acid and Sonic Hedgehog. Immunocytochemistry staining of the isolated OE-MSCs demonstrated their capability to express nestin and vimentin, as the two markers of primitive neuroectoderm. The motor neuron differentiation of OE-MSCs resulted in changing their morphology into bipolar cells with high expression of motor neuron markers of ChAT, Hb-9 and Islet-1 at the level of mRNA and protein. Consequently, we believe that the OE-MSCs have great potential to differentiate into motor neuron-like cells and can be an ideal stem cell source for the treatment of motor neuron-related disorders of central nervous system. © 2018 Elsevier B.V.
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