Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Differentiation of Human Olfactory System-Derived Stem Cells Into Dopaminergic Neuron-Like Cells: A Comparison Between Olfactory Bulb and Mucosa As Two Sources of Stem Cells Publisher Pubmed



Alizadeh R1 ; Ramezanpour F2 ; Mohammadi A2 ; Eftekharzadeh M2 ; Simorgh S2, 3 ; Kazemiha M2 ; Moradi F2
Authors
Show Affiliations
Authors Affiliations
  1. 1. ENT and Head & Neck Research Center and Department, The Five Senses Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Journal of Cellular Biochemistry Published:2019


Abstract

Cell transplantation has become a possible therapeutic approach in the treatment of neurodegenerative diseases of the nervous system by replacing lost cells. The current study aimed to make a comparison between the differentiation capacity of the olfactory bulb neural stem cells (OB-NSCs) and olfactory ectomesenchymal stem cells (OE-MSCs) into dopaminergic-like neurons under the inductive effect of transforming growth factor β (TGF-β). After culturing and treating with TGF-β, the differentiation capacities of both types of stem cells into dopaminergic neuron-like cells were evaluated. Quantitative real-time polymerase chain reaction analysis 3 weeks after induction demonstrated that the mRNA expression of the dopaminergic activity markers tyrosine hydroxylase (TH), dopamine transporter (DAT), paired box gene 2 (PAX2), and PAX5 in the neuron-like cells derived from OB-NSCs was significantly higher than those derived from OE-MSCs. These findings were further supported by the immunocytochemistry staining showing that the expression of the tyrosine hydroxylase, DAT, PAX2, and paired like homeodomain 3 seemed to be slightly higher in OB-NSCs compared with OE-MSCs. Despite the lower differentiation capacity of OE-MSCs, other considerations such as a noninvasive and easier harvesting process, faster proliferation attributes, longer life span, autologous transplantability, and also the easier and inexpensive cultural process of the OE-MSCs, cumulatively make these cells the more appropriate alternative in the case of autologous transplantation during the treatment process of neurodegenerative disorders like Parkinson's disease. © 2019 Wiley Periodicals, Inc.
Related Docs
View other Related Docs
1. In Vitro Differentiation of Neural Stem Cells Derived From Human Olfactory Bulb Into Dopaminergic-Like Neurons, European Journal of Neuroscience (2017)
2. Differentiation of Neural Crest Stem Cells From Nasal Mucosa Into Motor Neuron-Like Cells, Journal of Chemical Neuroanatomy (2018)
3. Promoting Motor Functions in a Spinal Cord Injury Model of Rats Using Transplantation of Differentiated Human Olfactory Stem Cells: A Step Towards Future Therapy, Behavioural Brain Research (2021)
4. Stem Cell-Based Approach for the Treatment of Parkinson's Disease, Medical Journal of the Islamic Republic of Iran (2015)
5. Human Umbilical Cord–Derived Mesenchymal Stem Cells Into Oligodendrocyte-Like Cells Using Triiodothyronine As an Inducer: A Rapid and Efficient Protocol, Regenerative Engineering and Translational Medicine (2023)
6. Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering, Materials (2021)
7. Impact of Exosome-Loaded Chitosan Hydrogel in Wound Repair and Layered Dermal Reconstitution in Mice Animal Model, Journal of Biomedical Materials Research - Part A (2020)
8. Copper-Enriched Diamond-Like Carbon Coatings Promote Regeneration at the Bone–Implant Interface, Heliyon (2020)
Experts (# of related papers)
View all Related Experts
Mahmoud Reza Hadjighassem (3)
Sina Mojaverrostami (2)
Other Related Docs
9. Thermosensitive Chitosan/Poly(N-Isopropyl Acrylamide) Nanoparticles Embedded in Aniline Pentamer/Silk Fibroin/Polyacrylamide As an Electroactive Injectable Hydrogel for Healing Critical-Sized Calvarial Bone Defect in Aging Rat Model, International Journal of Biological Macromolecules (2022)
10. Mesenchymal Stem Cell Spheroids Embedded in an Injectable Thermosensitive Hydrogel: An in Situ Drug Formation Platform for Accelerated Wound Healing, ACS Biomaterials Science and Engineering (2020)
11. Gender and Age Related Changes in Number of Dopaminergic Neurons in Adult Human Olfactory Bulb, Journal of Chemical Neuroanatomy (2015)
12. Chitosan/Polyvinyl Alcohol Nanofibrous Membranes: Towards Green Super-Adsorbents for Toxic Gases, Heliyon (2019)
13. Sustained Delivery of Olanzapine From Sunflower Oil-Based Polyol-Urethane Nanoparticles Synthesised Through a Cyclic Carbonate Ring-Opening Reaction, IET Nanobiotechnology (2019)
14. Cross-Linked Polysaccharides in Drug Delivery, Tailor-Made Polysaccharides in Drug Delivery (2022)
15. Poloxamer: A Versatile Tri-Block Copolymer for Biomedical Applications, Acta Biomaterialia (2020)
16. Pcl/Gelatin Scaffolds and Beta-Boswellic Acid Synergistically Increase the Efficiency of Cgr8 Stem Cells Differentiation Into Dopaminergic Neuron: A New Paradigm of Parkinson's Disease Cell Therapy, Journal of Biomedical Materials Research - Part A (2021)
17. Agarose-Based Biomaterials: Opportunities and Challenges in Cartilage Tissue Engineering, Polymers (2020)
18. Agarose-Based Biomaterials for Advanced Drug Delivery, Journal of Controlled Release (2020)
19. Engineering the Niche for Hair Regeneration — a Critical Review, Nanomedicine: Nanotechnology# Biology# and Medicine (2019)
20. From Microporous to Mesoporous Mineral Frameworks: An Alliance Between Zeolite and Chitosan, Carbohydrate Research (2020)
21. Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering, Biomolecules (2019)
22. Grafted Polysaccharides in Drug Delivery, Tailor-Made Polysaccharides in Drug Delivery (2022)
23. Conductive Biomaterials As Nerve Conduits: Recent Advances and Future Challenges, Applied Materials Today (2020)
24. Injectable Cell-Laden Hydrogels for Tissue Engineering: Recent Advances and Future Opportunities, Tissue Engineering - Part A (2021)
25. Turning Toxic Nanomaterials Into a Safe and Bioactive Nanocarrier for Co-Delivery of Dox/Pcrispr, ACS Applied Bio Materials (2021)
26. Umbilical Cord-Derived Mesenchymal Stem Cells in Neurodegenerative Disorders: From Literature to Clinical Practice, Regenerative Medicine (2020)
27. Thermo-Sensitive Polymers in Medicine: A Review, European Polymer Journal (2019)