Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Cardioprotective Effects of the Aqueous Extract of Echinops Cephalotes on Myocardial Ischemia-Reperfusion in Rats by Modulation of Mmp-2, Mmp-9, Timp, and Oxidative Stress Publisher Pubmed



Fallah M1 ; Rakhshan K2 ; Nikbakht F1 ; Malekiravasan N3 ; Tahghighi A4 ; Azizi Y1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Laboratory of Medicinal Chemistry, Department of Clinical Research, Pasteur Institute of Iran, Tehran, Iran

Source: Biomedicine and Pharmacotherapy Published:2024


Abstract

Echinops plants have received great attention for the treatment of many diseases due to pharmacological properties such as their antidiabetic, antioxidant, and anti-inflammatory characteristics. The major purpose of the present study was to investigate the cardioprotective benefits of Echinops cephalotes (Ech) against myocardial ischemia-reperfusion (MI/R) injury. Male Wistar rats were randomly allocated to three groups: sham, MI, and MI + Ech. The left coronary artery (LAD) was blocked for 30 minutes to induce MI. In the treatment group, rats were given 150 mg/kg/day of Ech extract for 28 days. Aqueous extracts were made from Echinops plants. To study heart function, fibrosis, cardiac damage indicators, and oxidative stress factors, echocardiography, Masson's trichrome staining, and biochemical tests were used. The expression of matrix metalloproteinase 2 and 9 (MMP2 and MMP-9) and tissue inhibitor of metalloproteinase (TIMP) was determined using Western blotting. Tissue damage was assessed using hematoxylin and eosin staining. MI group exhibited significantly reduced ejection fraction (EF) and fractional shortening (FS), enhanced levels of lactate dehydrogenase (LDH), creatine kinase MB (CK-MB), cardiac Troponin I (cTnI), and malondialdehyde (MDA), as well as a decrease in the Glutathione (GSH) tissue content, reduced activity of superoxide dismutase (SOD), increasing fibrosis, upregulations of MMP-2 and MMP-9, and reduction of TIMP compared to the sham group. The findings suggest that Ech in particular, could be a promising therapeutic agent to reduce the damage in MI by targeting oxidative stress and modulating the activities of matrix metalloproteinases and their tissue inhibitors. © 2024 The Authors
Related Docs
1. Regulatory Effects of Trimetazidine in Cardiac Ischemia/Reperfusion Injury, Naunyn-Schmiedeberg's Archives of Pharmacology (2023)