Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation

Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation Publisher

Nekounam H¹ ; Samadian H² ; Golmohammadi H³ ; Asghari F^{1, 6} ; Shokrgozar MA⁴ ; Ahadian S⁵ ; Majidi RF¹

Show Affiliations

1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3. Department of Engineering, Islamic Azad University of South-Tehran Branch, Tehran, Iran
4. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
5. NouBio Inc., Los Angeles, United States
6. Department Of Internal Medicine, Yale University School Of Medicine, New Haven, CT, United States

Source: Surfaces and Interfaces Published:2023

Abstract

Engineered nanostructures are innovative and precisely designed, synthesized, and tailored with outstanding physicochemical properties that can be applied as the game-changer in neural tissue engineering. The present study aimed to develop an innovative approach based on electrical stimulation through a conductive scaffold to differentiate neural cells from human adipose mesenchymal stem cells without the use of a specific environment for neural differentiation. Electrospun carbon nanofibers (CNFs) were obtained using heat treatment of polyacrylonitrile nanofibers and treated by nitric acid, ethylenediamine, and oxygen Plasma. SEM imaging revealed that the treated nanofibers have s diameter in the range of 120–200 nm and the treatment did not significantly change the CNFs diameter. The FTIR results showed that the treatments were able to introduce COOH, OH, and NH2 functional groups on the CNFS surface. The XRD and Raman analysis showed that the plasma treatment induced the lowest structural changes in the CNFs microstructure. The biocompatibility assessments showed that the pristine and treated CNFs were nontoxic induced proliferative effect on human adipose-derived mesenchymal stem cells. The electrical stimulation (1.5 mA current with a frequency of 500 Hz and CMOS waveform for 7 days 10 min each day) induced the expression of neural genes and proteins by the cells cultured on the treated CNFs. The Plasma-treated CNFs mediated the highest differentiation outcome. These results indicate that electrospun CNFs can be applied as the innovative interface applicable for neural tissue regeneration under electrical stimulation. © 2023

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Reza Faridi Majidi (9)

Zahra Hassannejad (1)

Style	Citing Format
MLA	Nekounam H, et al.. "Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation." Surfaces and Interfaces, vol. 40, no. , 2023, pp. -.
APA	Nekounam H, Samadian H, Golmohammadi H, Asghari F, Shokrgozar MA, Ahadian S, Majidi RF (2023). Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation. Surfaces and Interfaces, 40(), -.
Chicago	Nekounam H, Samadian H, Golmohammadi H, Asghari F, Shokrgozar MA, Ahadian S, Majidi RF. "Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation." Surfaces and Interfaces 40, no. (2023): -.
Harvard	Nekounam H et al. (2023) 'Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation', Surfaces and Interfaces, 40(), pp. -.
Vancouver	Nekounam H, Samadian H, Golmohammadi H, Asghari F, Shokrgozar MA, Ahadian S, et al.. Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation. Surfaces and Interfaces. 2023;40():-.
BibTex	@article{ author = {Nekounam H and Samadian H and Golmohammadi H and Asghari F and Shokrgozar MA and Ahadian S and Majidi RF}, title = {Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation}, journal = {Surfaces and Interfaces}, volume = {40}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Nekounam H AU - Samadian H AU - Golmohammadi H AU - Asghari F AU - Shokrgozar MA AU - Ahadian S AU - Majidi RF TI - Carbon Nanofibers Fabrication, Surface Modifications, and Application As the Innovative Substrate for Electrical Stimulation of Neural Cell Differentiation JO - Surfaces and Interfaces VL - 40 IS - SP - EP - PY - 2023 ER -

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