Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Enhanced Transient Expression of an Anti-Cd52 Monoclonal Antibody in Cho Cells Through Utilization of Mirna Sponge Technology Publisher



Pairawan MS1 ; Bolhassani A2 ; Rahimpour A3, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Medical Nano-Technology and Tissue Engineering Research Center, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Research in Pharmaceutical Sciences Published:2019


Abstract

Chinese hamster ovary (CHO) cells are the dominant mammalian host system for the production of recombinant therapeutic proteins. Improving the viable cell density during culture of recombinant CHO cells can greatly affect the production yield. MicroRNAs (miRs) -15a and 16-1 are known as negative regulators of multiple genes involved in cell cycle progression and apoptotic inhibition. miR sponges, which act as decoy targets, are transcripts which contain complementary binding sites to the seed region of related miRs. Stably expressed miR sponges are known as efficient tools for miR loss of function studies. In this study, stable CHO cell pools and clones expressing miRs-15a and 16-1 specific decoy transcript downstream of an enhanced green fluorescent protein reporter gene was developed. Analysis of cell growth during 12 days of batch culture indicated improved maximum viable cell density of CHO cells and clones expressing the decoy transcript. In addition, transient expression of a recombinant anti-CD52 monoclonal antibody was significantly improved in a decoy harboring CHO cell clone, representing a 3.37-fold increase in yield after 4 days of culture. Our results indicated that miR sponge technology can be successfully applied for the improvement of cell viability and transient monoclonal antibody expression in CHO host cells. © 2019 Wolters Kluwer Medknow Publications. All rights reserved.
Related Docs
1. Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines, Applied Biochemistry and Biotechnology (2018)
2. Development of an Improved Lentiviral Based Vector System for the Stable Expression of Monoclonal Antibody in Cho Cells, Preparative Biochemistry and Biotechnology (2019)
3. Crispr-Interceded Cho Cell Line Development Approaches, Biotechnology and Bioengineering (2023)
4. Overexpression of Microrna-16 Declines Cellular Growth, Proliferation and Induces Apoptosis in Human Breast Cancer Cells, In Vitro Cellular and Developmental Biology - Animal (2015)
5. Role of Micrornas in Chronic Lymphocytic Leukemia Pathogenesis, Current Medicinal Chemistry (2020)
6. The Augmenting Effects of the Tdna Insulator on Stable Expression of Monoclonal Antibody in Chinese Hamster Ovary Cells, Monoclonal Antibodies in Immunodiagnosis and Immunotherapy (2018)
7. Microfluidics for Detection of Exosomes and Micrornas in Cancer: State of the Art, Molecular Therapy Nucleic Acids (2022)
8. Downregulation of Mir-542-3P Contributes to Apoptosis Resistance in Dermal Fibroblasts From Systemic Sclerosis Patients Via Survivin Overexpression, Iranian Journal of Allergy# Asthma and Immunology (2019)
Experts (# of related papers)
View all Related Experts
Hamid Reza Mirzaei (1)
Mohammad Reza Khorramizadeh (1)