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Fabrication of Cancellous Biomimetic Chitosan-Based Nanocomposite Scaffolds Applying a Combinational Method for Bone Tissue Engineering Publisher Pubmed



Jamalpoor Z1 ; Mirzadeh H2, 3 ; Joghataei MT2 ; Zeini D3 ; Bagherikhoulenjani S3 ; Nourani MR4
Authors
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Authors Affiliations
  1. 1. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), P.O. Box 1449614535, Tehran, Iran
  2. 2. Cellular and Molecular Medical Research Center, Iran University of Medical Science, P.O. Box 1449614535, Tehran, Iran
  3. 3. Polymer Engineering Department, Amirkabir University of Technology, P.O. Box 15875/4413, Tehran, Iran
  4. 4. Tissue Engineering Division, Biotechnology Research Center, Baqiyatallah University of Medical Sciences, P.O. Box 14359-44711, Tehran, Iran

Source: Journal of Biomedical Materials Research - Part A Published:2015


Abstract

The aim of this study was to mimic the specific structure of bone and fabricate a biomimetic nano-hydroxyapatite (HA)/chitosan (Cs)/gelatin scaffolds using combination of particle leaching and freeze drying methods eliminating mold effects. To achieve an optimum structure, scaffolds with different gelatin/Cs weight ratio were fabricated. Morphological characterization of scaffolds by scanning electron microscopy method showed highly interconnected porous structures similar to cancellous bone with mean pore size ranging from 140 to 190 μm. Nano-HA crystals were dispersed homogeneously in the polymer matrix according to the energy-dispersive X-ray spectroscopy and transmission electron microscopy images. Fourier transform infrared and X-ray diffraction results disclosed that chemical interactions were formed between nano-HA, Cs, gelatin and crystallinity of each material decreased with blending. It was found that increasing the gelatin content significantly improved water uptake, degradation rate as well as attachment, infiltration and proliferation of Saos2 cells to the scaffolds. The presented results confirm that the designed biomimetic nano-HA /Cs/gelatin scaffolds can be used as promising substitutes for bone tissue engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1882-1892, 2015. © 2014 Wiley Periodicals, Inc.
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