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Carboxymethyl Chitosan/Gelatin/Hydroxyapatite Biomimetic Cryogels for Bone Regeneration Publisher



Asadi B1 ; Mirzadeh H1 ; Olov N1 ; Samadikuchaksaraei A2 ; Kheirbakhsh R3 ; Moradi R4 ; Amanpour S3 ; Bagherikhoulenjani S1
Authors
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Authors Affiliations
  1. 1. Department of Polymer and Colour Engineering, Amirkabir University of Technology, Tehran, Iran
  2. 2. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Orthopedic Hand Surgeon, Naft Hospital, Tehran, Iran

Source: Bioinspired# Biomimetic and Nanobiomaterials Published:2023


Abstract

Gelation at a temperature below the freezing point of a solvent is known as cryogelation, which has attracted attention in regenerative medicine due to its improved mechanical and structural properties. This study focuses on using cryogelation as a versatile method for fabricating biomimetic scaffolds with improved mechanical strength and larger pore sizes with potential for bone tissue engineering applications. Scaffolds comprising carboxymethyl chitosan/gelatin/nano-hydroxyapatite were formed through cryogelation. The effects of three parameters-namely, cross-linker concentration, mineral content and gelatin-To-polymer ratio-on physical, chemical and mechanical properties were investigated. In this work, MG63 cells were used for an in vitro assay. In addition, an in vivo assay was conducted to investigate the biocompatibility of the nanocomposite scaffolds. The results showed that all scaffolds have a porous structure with interconnected pores with a morphology similar to bone structure. Their pore size, porosity and swelling ratio decreased with an increased cross-linker concentration, while nano-hydroxyapatite had the opposite effect. X-ray diffraction results also showed that the inorganic phase retained its crystallinity in the substrate with a slight decrease in crystal size. In addition, the scaffolds showed no toxicity in either in vitro or in vivo studies. The obtained results showed that biomimetic cryogels based on carboxymethyl chitosan/gelatin/hydroxyapatite have potential for bone tissue engineering. © 2023 ICE Publishing: All rights reserved.
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