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The Study of Phenotypic and Genotypic Importance of Fungal Isolated From Traditional Milk and Cheese in Iran Publisher



Faghihi Shahrestani F1 ; Tajabadi Ebrahimi M1 ; Bayat M1 ; Hashemi J2 ; Razavilar V3
Authors
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Authors Affiliations
  1. 1. Department of Pathobiology, faculty of veterinary‘ specialized science, Science and Research Branch, Islamic Azad university, Tehran, Iran
  2. 2. Tehran University of Medical Science, Tehran, Iran
  3. 3. Department of Food of Microbiology, Faculty of Veterinary Specialized Science, Science and Research Branch, Tehran Islamic Azad university, Tehran, Iran

Source: Gene Reports Published:2019


Abstract

Fungi, in particular some of the Penicillium and Aspergillus species, are the main cause of dairy products contamination, they threaten the general health of community and food safety due to the production of mycotoxins. The purpose of this study was to isolate and identify saprophytic fungi contaminating various dairy products in Shiraz in comparison to the National Iranian Standard (2406). In this study, 85 samples (40 cheeses, and 45 milk samples) were collected from Shiraz city of Iran and analyzed for the presence of mold and yeast. After morphological investigation, molecular tests was done by PCR method. The results were compared to the National Iranian Standard and were analyzed by SPSS version 20 and chi-square statistical analysis with about 95% accuracy and significant level (p-value<0.00001). Among the total of 85 samples, 15 (17.6%) samples showed contamination more than allowed limit. After molecular tests and sequencing of ITS region and D1/D2; Saccharomyces cerevisiae, Saccharomyces recorded in NBCI center. According to the results of this study, it is necessary to take serious measures to control the contamination of fungal dairy products without the use of preservatives. © 2019