Simultaneous Coating of Electrospun Nanofibers With Bioactive Molecules for Stem Cell Osteogenesis in Vitro Publisher



Zahiritoosi M¹ ; Zargar SJ² ; Seyedjafari E³ ; Saberian M⁴ ; Ahmadi M²
Source: Cell Journal Published:2024

Abstract

Objective: Mesenchymal stem cells (MSCs) are widely recognized as a promising cell type for therapeutic applications due to their ability to secrete and regenerate bioactive molecules. For effective bone healing, it is crucial to select a scaffold that can support, induce, and restore biological function. Evaluating the scaffold should involve assessing MSC survival, proliferation, and differentiation. The principal aim of this investigation was to formulate composite nanofibrous scaffolds apt for applications in bone tissue engineering. Materials and Methods: In this experimental study, nanofibrous scaffolds were fabricated using Poly-L-lactic acid (PLLA) polymer. The PLLA fibers' surface was modified by integrating collagen and hydroxyapatite (HA) nanoparticles. Results: The findings demonstrated that the collagen- and nanohydroxyapatite-modified electrospun PLLA scaffold positively influenced the attachment, growth, and osteogenic differentiation of MSCs. Conclusion: Coating the nanofiber scaffold with collagen and nanoparticle HA significantly enhanced the osteogenic differentiation of MSCs on electrospun PLLA scaffolds. © 2024 Royan Institute (ACECR). All rights reserved.