Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Potential Role of Human Chorionic Gonadotropin Supplementation in Spermatogenesis in Rats Subjected to Forced Swimming Exercise Publisher Pubmed



Moayeri A1 ; Darvishi M1 ; Hamidabadi HG2 ; Mojaverrostami S3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Ilam University of Medical Sciences, Ilam, Iran
  2. 2. Mazandaran University of Medical Sciences, Sari, Iran
  3. 3. Tehran University of Medical Sciences, Tehran, Iran

Source: Folia medica Published:2021


Abstract

AIM: The aim of this study was to evaluate the supportive effect of human chorionic gonadotropin (hCG) on the quality of spermatogenesis, including count, motility, morphology, viability and apoptosis of sperm following forced swimming exercise. MATERIALS AND METHODS: Twenty-four adult male Sprague-Dawley rats were used in this study. All rats were divided into four groups: control group; swimming exercise group (S); hCG administration group and swimming (SG) with hCG administration group (G). The experimental group was trained to force swimming stress for 10 min for 6 days. Then the sperm quality parameters were measured after dissection and epididymis removal. Spermatogenesis and germ cell apoptosis were evaluated by using Miller & Johnsen's score and TUNEL staining respectively. RESULTS: Results showed the count (control: 113±3.1, S: 74±1.9, G: 111±3, and SG: 103±2.4), motility (control:  93±2, S: 67±2.8,G: 90±2.7, and SG: 78±1), morphology (control: 89±3%, S: 47±2.4%, G: 90±3.1%, and SG: 67±1.1%), and viability of sperm (control: 91±2.9, S: 50±2, G: 91±1.9, and SG: 70±1.3) in swimming exercised-hCG administered group, significantly enhanced by hCG treatment compared to the swimming exercise group (p≤0.01). Also the number of apoptotic germ cells significantly decreased in swimming exercised-hCG administered group compared to the swimming exercised group. CONCLUSIONS: These results suggest that administration of hCG can protect the testes against the detrimental effect of forced swimming exercise in adult male rats. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.