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Simple and Effective Label Free Electrochemical Immunosensor for Fig Mosaic Virus Detection Publisher Pubmed



Hajihashemi H1 ; Safarnejad MR2 ; Norouzi P1, 3 ; Ebrahimi M1 ; Shahmirzaie M4 ; Ganjali MR1, 3
Authors
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Authors Affiliations
  1. 1. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
  2. 2. Department of Plant Viruses, Iranian Research Institute of Plant Protections, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
  3. 3. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran

Source: Analytical Biochemistry Published:2019


Abstract

Here, the construction and characterization of the first immunosensor for highly sensitive and label free detection of Fig mosaic virus (FMV) is reported. The specific antibody against nucleocapsid of the virus was raised and immobilized at the surface of 11-mercaptoundecanoic acid (MUA) and 3-mercapto propionic acid (MPA) modified gold electrode, via carbodiimide coupling reaction. The immunosensor fabrication steps were characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The electrochemical detection of FMV was conducted using differential pulse voltammetry in ferri/ferrocyanide solution as a redox probe. The proposed immunosensor exhibited high selectivity, good reproducibility and high sensitivity for FMV detection in a range from 0.1 nM to 1 μM with a detection limit of 0.03 nM. Moreover, good results were obtained for determination of FMV in real samples, indicating the feasibility of the developed immunosensor for detection of fig mosaic disease, without the need for molecular (e.g. PCR) amplification. © 2018