Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Pcl/Gelatin Scaffolds and Beta-Boswellic Acid Synergistically Increase the Efficiency of Cgr8 Stem Cells Differentiation Into Dopaminergic Neuron: A New Paradigm of Parkinson's Disease Cell Therapy Publisher Pubmed



Kheradmand H1 ; Babaloo H2 ; Vojgani Y3, 4 ; Mirzakhanlouei S5 ; Bayat N6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Neurosurgery, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  3. 3. Department of Molecular Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Student Research Committee, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Biotechnology Department, Faculty of Advanced Science and Technologies, University of Isfahan, Isfahan, Iran
  6. 6. Brain and Spinal Cord Injury Research Center (BASIR), Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Biomedical Materials Research - Part A Published:2021


Abstract

Parkinson's disease is a progressive degenerative disorder in the central nervous system, which is distinguished by the death of dopamine-producing nerve cells. Levodopa, a dopamine precursor drug, is the current standard of care of symptomatic treatment for Parkinson's disease. However, the long-term use of the drug is associated with the development of motor fluctuations and dyskinesias. Cellular therapies aim to deploy fetal dopaminergic neurons as a means to replace the missing dopamine-producing cells. The present study aims to study the impact of beta-boswellic acid (BBA) coupled with poly ε-caprolactone (PCL)/gelatin scaffolds on the dopaminergic differentiation course of CGR8 embryonic stem cells (ESCs). For this purpose, CGR8 ESCs were cultured on PCL/gelatin scaffolds and a five-step protocol was employed to be promoted the neural differentiation of CGR8 ESCs. Gene expression analysis by real-time qPCR demonstrated that PCL/gelatin scaffolds along with BBA treatment impose synergistic effects on the derivation of dopaminergic-like cells from CGR8 ESCs. Reverse-phase high-performance liquid chromatography confirmed the functionality of the derived neurons by demonstrating the efficient secretion of dopamine in response to stimuli. Our results suggested that the generation of functional dopaminergic-like cells from CGR8 ESCs was increased and supported by PCL/gelatin scaffolds and BBA treatment can heighten the efficiency. The result of this study may open insight into Parkinson's disease cell therapy and provide future directions for tissue engineering aimed at the treatment of Parkinson's disease. © 2020 Wiley Periodicals LLC
Related Docs
View other Related Docs
1. Directed Differentiation of Dopamine-Secreting Cells From Nurr1/Gpx1 Expressing Murine Embryonic Stem Cells Cultured on Matrigel-Coated Pcl Scaffolds, Molecular Neurobiology (2017)
2. Stem Cell-Based Approach for the Treatment of Parkinson's Disease, Medical Journal of the Islamic Republic of Iran (2015)
3. Pcl/Gelatin Nanofibrous Scaffolds With Human Endometrial Stem Cells/Schwann Cells Facilitate Axon Regeneration in Spinal Cord Injury, Journal of Cellular Physiology (2019)
4. Differentiation of Human Olfactory System-Derived Stem Cells Into Dopaminergic Neuron-Like Cells: A Comparison Between Olfactory Bulb and Mucosa As Two Sources of Stem Cells, Journal of Cellular Biochemistry (2019)
5. Therapeutic Potential of Stem Cells for Treatment of Neurodegenerative Diseases, Biotechnology Letters (2020)
6. Tissue-Engineered Regeneration of Hemisected Spinal Cord Using Human Endometrial Stem Cells, Poly Ε-Caprolactone Scaffolds, and Crocin As a Neuroprotective Agent, Molecular Neurobiology (2017)
7. Fabrication and Evaluation of Porous and Conductive Nanofibrous Scaffolds for Nerve Tissue Engineering, Journal of Materials Science: Materials in Medicine (2021)
8. In Vitro Differentiation of Neural Stem Cells Derived From Human Olfactory Bulb Into Dopaminergic-Like Neurons, European Journal of Neuroscience (2017)
Experts (# of related papers)
View all Related Experts
Zahra Hassannejad (1)
Somayeh Ebrahimi Barough (1)
Other Related Docs
9. Development of Biodegradable Electrospun Gelatin/Aloe-Vera/Poly(Ε‑Caprolactone) Hybrid Nanofibrous Scaffold for Application As Skin Substitutes, Materials Science and Engineering C (2018)
10. Tissue Engineering Scaffolds in the Treatment of Brain Disorders in Geriatric Patients, Artificial Organs (2019)
11. Fabrication of Poly(L-Lactic Acid)/Chitosan Scaffolds by Solid-Liquid Phase Separation Method for Nerve Tissue Engineering: An in Vitro Study on Human Neuroblasts, Journal of Craniofacial Surgery (2019)
12. Appropriate Scaffold Selection for Cns Tissue Engineering, Avicenna Journal of Medical Biotechnology (2020)