Dendrosome-Encapsulated Beta-Boswellic Acid Boosts Expression of the Memory-Related Genes in the B65 Cell Line Publisher



Khalajkondori M¹ ; Ahmadisani K¹ ; Hosseinzadeh A² ; Abtin M³ Show Affiliations
Source: Journal of Drug Delivery Science and Technology Published:2020

Abstract

A growing body of evidence suggests that beta-Boswellic acid (βBA), one of the main ingredients of Boswellia extract, could promote neuronal growth, synaptic plasticity and impacts the memory performance. Moreover, ingredients of the Boswellia extract are well-known due to their anti-inflammatory effects. However, the poor water solubility and bioavailability of Boswellic acids restricts their superior therapeutic profits. To overcome this limit, we encapsulated βBA by a micellar nanostructured particle named dendrosome, and characterized the dendrosomal βBA preparation. Scanning electron microscopy (SEM) analysis indicated that the dendrosomal βBA preparation comprised sphere-shape nanoparticles with a mean diameter of 155.1 ± 2.75 nm. Dynamic light scattering (DLS) identified a polydispersity index (PDI) of 0.056 and ζ-potential of 3.8 mV for the dendrosomal βBA nanoparticles. Cell viability analysis identified the 50% inhibitory concentration (IC50) values of 58.42 μM for 24 h, 44.87 μM for 48 h and 16.69 μM for 72 h in the B65 cells treated with the dendrosomal βBA. Comparison of the expression profiles of the five memory-related genes (CREB1, CREB2, BDNF, FMR1 and MAP1B) in the B65 cells treated with 0.1, 1 and 10 μM of the dendrosomal βBA and free βBA for 24 h, 48 h and 72 h time intervals, revealed that the impacts of the dendrosomal βBA on the genes expression levels were significantly higher than the free βBA. Additionally, in response to the dendrosomal βBA treatments, expression levels of CREB1, BDNF and FMR1 were significantly enhanced in the 24 h and 72 h time intervals, while they showed no significant changes in the 48 h, compared to the control. In fact, we observed an oscillated expression model for these genes. The expression profiles of the CREB2 and MAP1B were about opposite to the CREB1, BDNF and FMR1 profiles. In conclusion, these results indicated that the encapsulation of βBA by dendrosome nanoparticles might improve its uptake by the cells and enhance the impact of βBA on the memory performance. © 2020 Elsevier B.V.