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Molecular Characterization of Animal Fasciola Spp. Isolates From Kermanshah,Western Iran



Bozorgomid A1 ; Nazari N2 ; Rahimi H3 ; Beigom Kia E1 ; Hajjaran H1 ; Mohebali M1, 4 ; Aryaeipour M1 ; Heidarian P1 ; Ezati MS5 ; Rokni MB1, 4
Authors
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Authors Affiliations
  1. 1. Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Dept. of Medical Parasitology and Mycology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
  3. 3. Dept. of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Dept. of Food Hygiene, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

Source: Iranian Journal of Public Health Published:2016

Abstract

Background: We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep,cattle and goat from Kermanshah Province,western Iran using PCR-RFLP method. Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep,28 cattle,4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene. Results: In Kermanshah,F. hepatica was present in 96% of the samples,F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study. Conclusion: Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran. © 2016,Iranian Journal of Public Health. All rights reserved.
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