Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm)

Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm)

Moghadamizad Z¹ ; Hosseinisafa A¹ ; Mohebali M^{1, 2} ; Heydarian P³ ; Aryaeipour M¹ ; Rokni MB^{1, 2}

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1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
3. Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

Source: Iranian Journal of Public Health Published:2020

Abstract

Background: It is difficult to make an exact morphological distinction between Fasciola hepatica and Fasciola gigantica. We used High Resolution Melting analysis (HRM) method to differentiate the F. hepatica species from F. gigantica in order to differentiate them. Methods: Overall, 80 adult liver flukes were collected from infected slaughtered animals including cattle, sheep and goats from Lorestan Province, western Iran from Sep 2015 to Aug 2017. Genomic DNA was extracted using commercial DNA extraction kit. The multilocus sequences of mDNA including COX1, COX3 and ND6 were amplified employing real-time PCR & HRM analysis. Specific and universal primer pairs were designed for differentiation Fasciola spp. Results: Universal primers cannot be used to distinguish between these two species, but in the contrary, specific primer pairs of each species could differentiate them properly. Molecular identification using specific primer pairs were consistent. Conclusion: HRM is a simple, fast and reliable method for detecting and differentiating F. hepatica from F. gigantica and can be used for diagnostic and epidemiological purposes. © 2020, Iranian Journal of Public Health. All rights reserved.

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Style	Citing Format
MLA	Moghadamizad Z, et al.. "Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm)." Iranian Journal of Public Health, vol. 49, no. 3, 2020, pp. 521-529.
APA	Moghadamizad Z, Hosseinisafa A, Mohebali M, Heydarian P, Aryaeipour M, Rokni MB (2020). Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm). Iranian Journal of Public Health, 49(3), 521-529.
Chicago	Moghadamizad Z, Hosseinisafa A, Mohebali M, Heydarian P, Aryaeipour M, Rokni MB. "Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm)." Iranian Journal of Public Health 49, no. 3 (2020): 521-529.
Harvard	Moghadamizad Z et al. (2020) 'Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm)', Iranian Journal of Public Health, 49(3), pp. 521-529.
Vancouver	Moghadamizad Z, Hosseinisafa A, Mohebali M, Heydarian P, Aryaeipour M, Rokni MB. Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm). Iranian Journal of Public Health. 2020;49(3):521-529.
BibTex	@article{ author = {Moghadamizad Z and Hosseinisafa A and Mohebali M and Heydarian P and Aryaeipour M and Rokni MB}, title = {Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm)}, journal = {Iranian Journal of Public Health}, volume = {49}, number = {3}, pages = {521-529}, year = {2020} }
RIS	TY - JOUR AU - Moghadamizad Z AU - Hosseinisafa A AU - Mohebali M AU - Heydarian P AU - Aryaeipour M AU - Rokni MB TI - Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm) JO - Iranian Journal of Public Health VL - 49 IS - 3 SP - 521 EP - 529 PY - 2020 ER -

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