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Design and Manufacturing of Electromechanical Chip for Rapid Measurement of Igg1 Antibody in Cell-Culture Supernatant Publisher



Rahimpour A1, 2 ; Yazdian F3 ; Jonoush ZA4 ; Rajabibazl M5 ; Omidi M2
Authors
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Authors Affiliations
  1. 1. Medical Nano-Technology and Tissue Engineering Research Center, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Faculty of New Science and Technology University of Tehran, Tehran, Iran
  4. 4. Department of Immunology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Alem Sq., Safaieh, Iran
  5. 5. Department of Medical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Biology and Medicine Published:2016


Abstract

In this study, we have used arrays of piezoresistive nanomechanical membrane resonators (NMRs) to detect IgG1 antibody. The NMR consists of a membrane suspended by four piezoresistive sensing components. Unlike conventional cantilever mass sensors, our sensors retain a uniform mass sensitivity over the membrane surface. According to the experiments, it was revealed that NMRs have mass sensitivities in the order of 25 Hz/pg in air and 1.8 Hz/pg in liquid. This matter allows them to detect IgG1 over a wide range of concentrations from 100 pg/mL to 10 μg/mL in a background of human serum albumin (HSA) at 1 mg/ml. This indicates the fact that the self-sensing NMR approach is beneficial for detecting disease markers. Moreover, the performance of the NMR was compared with other detection methods, and the results indicated a better performance for NMR. © 2016 Omidi et al.
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