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Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in Hiv Patients



Shokri F1 ; Abbaszadeh R2 ; Rostamnezhad M3 ; Heidari Digesara S4 ; Marandi N5 ; Bairami Kuzehkanan A2 ; Abolhassani M6 ; Gharavi MJ2
Authors
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Authors Affiliations
  1. 1. Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Parasitology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
  3. 3. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Radiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  5. 5. Department of Medical Parasitology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  6. 6. Department Of Epidemiology, School of Public Health, Iran University of Medical Sciences, Tehran, Iran

Source: Journal of Babol University of Medical Sciences Published:2019

Abstract

BACKGROUND AND OBJECTIVE: Toxoplasma gondii is associated with several complications including neurological problems, ocular damage and encephalitis in immunodeficiency individuals. Early diagnosis of this infection can lead to better management of this disease. Therefore, this study was conducted to determine the presence of Toxoplasma gondii with two serologic and molecular methods in HIV-infected individuals. METHODS: In this cross-sectional study, 102 male patients with HIV with a mean age of 40 ± 9.2 years were examined. The serum sample was used for ELISA to determine the acute and chronic phase and cellular samples using Real Time-PCR for determining the acute phase of the disease. The relationship between age groups and the HIV transmission pathway, as well as the age group, was compared with the results of the Toxoplasma gondii test. FINDINGS: Out of 102 samples tested for IgM anti-Toxoplasma gondii antigen by ELFA, all (100%) samples were negative, but for anti-IgG anti-parasite, 44 samples (43.1%) were positive and 58 Sample (56.9%) was negative. Out of 102 samples tested by RT-PCR, all (100%) samples were negative for Toxoplasma DNA. There was a statistically significant relationship between age groups and transmission pathways (p<0.001), as well as between age groups with anti-Toxoplasma gondii IgG levels (p<0.001). CONCLUSION: According to the results of this study, the use of IgM-ELFA and PCR-RT methods for the diagnosis of acute phase and IgG-ELFA in the chronic phase of the disease is important. With the diagnosis of chronic form of toxoplasmosis, preventive treatments can be used in HIV+ patients. © 2019, Babol University of Medical Sciences. All rights reserved.
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