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Cytotoxic Evaluation of Daphne Pontica L. Aerial Part Extracts on Three Cancerous Cell Lines by Mtt Assay



Eskandari B1 ; Safavi M2 ; Lamardi SNS3 ; Vazirian M1, 4
Authors
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Authors Affiliations
  1. 1. Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
  3. 3. Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Traditional and Integrative Medicine Published:2019

Abstract

Nowadays, cancer is the second prevalent cause of mortality after cardiovascular diseases in developed and the third one in developing countries. Adverse effects of chemotherapeutic agents bring the necessity of investigating about new medications with fewer side effects. Daphne L. genus is one of the natural sources with valuable reported anticancer effects. This study aimed to assess the cytotoxic effect of some extracts from the aerial parts of Daphne pontica collected from North of Iran on cancer cell lines.Extraction of the plant material was performed by maceration (3×72 h) of 200 g of sample with petroleum ether, chloroform, ethyl acetate, and methanol, respectively. The total extract was also obtained by maceration of the sample with 80% ethanol. Different concentrations of the dried extracts were prepared to assess their cytotoxic effect by 24 h incubation of cell lines with different extracts and then MTT (dimethyl thiazolyl diphenyl tetrazolium) assay on three cancerous cell lines (MDA-MB-231, MCF-7 and T47D), performed in triplicate. IC50 was then estimated from curves constructed by plotting cell survival (%) versus sample concentration (μg/ml). Results indicated that ethyl acetate fraction of D. pontica had the most potent cytotoxic effect in MTT assay with IC50= 977.46 μg/ml; while other fractions were weaker in toxicity (IC50>1000 μg/ml). By comparing to potent cytotoxic effects of other Daphne species, it seems that the cytotoxic properties of D. pontica is different from other species of this genus since according to this study, no significant antineoplastic properties against the three breast cancer cell lines were determined. Further studies on other pharmacological activities of this plant are recommended. © 2019 Traditional and Integrative Medicine.