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Cytotoxic Effects of Eupatorium Cannabinum on Mcf-7 Human Breast Cancer Cell Line Publisher



Abuali M1 ; Ardekani MRS1 ; Rezadoost H2 ; Vazirian M1 ; Balaeikahnamoei M1 ; Hamzeloomoghadam M3
Authors
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Authors Affiliations
  1. 1. Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences, Tehran, Iran
  2. 2. Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
  3. 3. Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Research Journal of Pharmacognosy Published:2021


Abstract

Background and objectives: Cancer is known to be the second cause of death around the world. The most prevalent cancer among women is breast cancer. Use of plant-derived products in cancer treatment may reduce adverse side effects. Extensive research around the world has led to the discovery of herbal compounds that can be used to treat some types of cancer. According to previous studies, the methanol extract of Eupatorium cannabinum has shown cytotoxic effects in some cancer cell lines. In the present study, bioassay guided fractionation and isolation was conducted on E. cannabinum to evaluate the cytotoxic activity in MCF-7 breast cancer cell line. Methods: The Extraction from the aerial parts was performed by maceration method. Isolation and purification of the extracts were performed by column chromatography. The cytotoxic activity of different extracts of E. cannabinum was evaluated against MCF-7 cell line by MTT assay and a compound was isolated according to bioassay guided fractionation. The cytotoxic activity and apoptotic property of the isolated compound was determined. Results: The chloroform extract was the most active one with IC50 of 21.39±3.24 µg/mL followed by the n-hexane and methanol extracts with IC50values of 60.23±2.16 µg/mL and 81.74±3.41 µg/mL, respectively. IC50 of subfractions (1-6) from the chloroform extract were 60.83±2.56 µg/mL, 58.93±2.73 µg/mL, 37.5±3.65 µg/mL, 7.86±1.34 µg/mL, 10.61±2.34 µg/mL and 13.77±4.17 µg/mL, respectively. Eucannabinolide, a sesquiterpene lactone, was isolated from the chloroform extract according to bioassay guided fractionation. Its IC50 was found to be 13±2.45 µg/mL. Eucannabinolide induced 46.91% apoptosis at concentration of 13 µg/mL in MCF-7 cell line in Annexin V/PI assay. Conclusion: Eucannabinolideis a promising candidate for further breast cancer drug discovery studies. © 2021, Iranian Society of Pharmacognosy. All rights reserved.