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On-Chip Synthesis of Fine-Tuned Bone-Seeking Hybrid Nanoparticles Publisher Pubmed



Hasanisadrabadi MM1, 2, 3 ; Dashtimoghadam E4 ; Bahlakeh G5 ; Majedi FS3, 6 ; Keshvari H3 ; Van Dersarl JJ2 ; Bertsch A2 ; Panahifar A7 ; Renaud P2 ; Tayebi L4, 8 ; Mahmoudi M9, 10 ; Jacob KI1
Authors
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Authors Affiliations
  1. 1. Parker H Petit Institute for Bioengineering and Bioscience, GW Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, 30332-0295, GA, United States
  2. 2. Laboratoire de Microsystemes (LMIS4), Institute of Microengineering, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland
  3. 3. Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
  4. 4. Department of Developmental Sciences, Marquette University, School of Dentistry, Milwaukee, 53201, WI, United States
  5. 5. Department of Engineering and Technology, Golestan University, AliabadKatool, Iran
  6. 6. Department of Bioengineering, University of California at Los Angeles, Los Angeles, 951600, CA, United States
  7. 7. Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
  8. 8. Department of Engineering Science, University of Oxford, Oxford, OX13PJ, United Kingdom
  9. 9. Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  10. 10. Division of Cardiovascular Medicine, School of Medicine, Stanford University, Stanford, 94305, CA, United States

Source: Nanomedicine Published:2015


Abstract

Aims: Here we report a one-step approach for reproducible synthesis of finely tuned targeting multifunctional hybrid nanoparticles (HNPs). Materials & methods: A microfluidic-assisted method was employed for controlled nanoprecipitation of bisphosphonate-conjugated poly(D,L-lactide-co-glycolide) chains, while coencapsulating superparamagnetic iron oxide nanoparticles and the anticancer drug Paclitaxel. Results: Smaller and more compact HNPs with narrower size distribution and higher drug loading were obtained at microfluidic rapid mixing regimen compared with the conventional bulk method. The HNPs were shown to have a strong affinity for hydroxyapatite, as demonstrated in vitro bone-binding assay, which was further supported by molecular dynamics simulation results. In vivo proof of concept study verified the prolonged circulation of targeted microfluidic HNPs. Biodistribution as well as noninvasive bioimaging experiments showed high tumor localization and suppression of targeted HNPs to the bone metastatic tumor. Conclusion: The hybrid bone-targeting nanoparticles with adjustable characteristics can be considered as promising nanoplatforms for various theragnostic applications. © 2015 Future Medicine Ltd.
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