Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Tumor-Suppressing and Anti-Angiogenic Activities of a Recombinant Anti-Cd3ϵ Nanobody in Breast Cancer Mice Model Publisher Pubmed



Khatibi AS1 ; Roodbari NH1 ; Majidzadea K2 ; Yaghmaei P1 ; Farahmand L2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Recombinant Proteins Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran

Source: Immunotherapy Published:2019


Abstract

Achievements in cancer immunotherapy require augmentation of a host's anti-tumor immune response for anti-cancer modality. Materials & methods: Different concentrations of recombinant anti-CD3 nanobody were administered at predetermined time intervals during a 24-day treatment period and then expression of angiogenic biomarkers including VEGFR2, MMP9 and CD31, as well as tumor cell proliferation marker ki67, was determined in tumor sections by immunohistochemistry. Furthermore, expression of cytokines was examined in peripheral blood of mice. Results: Based on our results, administration of nanobody could reduce biomarker expression in tumor sections. Tumor growth was also delayed and survival rate was increased in response to nanobody treatment. Moreover, expression of pro-inflammatory cytokines was reduced. Conclusion: In conclusion, we demonstrated that administration of nanobody could effectively suppress angiogenesis as well as tumor growth. © 2019 © 2019 Future Medicine Ltd.
Related Docs
View other Related Docs
1. Engineered Hypoxia-Responding Escherichia Coli Carrying Cardiac Peptide Genes, Suppresses Tumor Growth, Angiogenesis and Metastasis in Vivo, Journal of Biological Engineering (2021)
2. Evaluation the Potential of Recombinant Anti-Cd3 Nanobody on Immunomodulatory Function, Molecular Immunology (2020)
3. Recombinant Nanobody Against Muc1 Tandem Repeats Inhibits Growth, Invasion, Metastasis, and Vascularization of Spontaneous Mouse Mammary Tumors, Molecular Oncology (2022)
4. Immunology of Cutaneous Tumors and Immunotherapy for Melanoma, Cancer Immunology: Cancer Immunotherapy for Organ-Specific Tumors (2015)
5. Extraction of Vessel Structure in Thermal Images to Help Early Breast Cancer Detection, Computer Methods in Biomechanics and Biomedical Engineering: Imaging and Visualization (2020)
6. A Fibroblast Growth Factor Antagonist Peptide Inhibits Breast Cancer in Balb/C Mice, Open Life Sciences (2018)
7. Interactive Effect of 6 Weeks of High-Intensity Interval Training and Quercetin Supplementation on Expression of Some Tumor Angiogenesis Markers in Female Mice With Breast Cancer, Medical Journal of Tabriz University of Medical Sciences (2021)
8. The Significant Role of Micrornas in Gliomas Angiogenesis: A Particular Focus on Molecular Mechanisms and Opportunities for Clinical Application, Cellular and Molecular Neurobiology (2023)
Experts (# of related papers)
View all Related Experts
Nima Rezaei (3)
Saeid Amanpour (1)
Other Related Docs
9. Controversial Role of Γδ T Cells in Pancreatic Cancer, International Immunopharmacology (2022)
10. Targeting Hypoxia and Hypoxia-Inducible Factor-1 in the Tumor Microenvironment for Optimal Cancer Immunotherapy, Journal of Cellular Physiology (2022)
11. In Vitro Inhibition of Angiogenesis by Heat and Low Ph Stable Hydroalcoholic Extract of Peganum Harmala Seeds Via Inhibition of Cell Proliferation and Suppression of Vegf Secretion, Pharmaceutical Biology (2015)
12. The Role of Interferon-Gamma and Its Receptors in Gastrointestinal Cancers, Pathology Research and Practice (2023)
13. The Role of T Helper 17 and Regulatory T Cells in Tumor Microenvironment, Immunopharmacology and Immunotoxicology (2019)
14. Molecular Mechanisms of Curcumins Suppressing Effects on Tumorigenesis, Angiogenesis and Metastasis, Focusing on Nf-Κb Pathway, Cytokine and Growth Factor Reviews (2016)
15. T Cell Engineered With a Novel Nanobody-Based Chimeric Antigen Receptor Against Vegfr2 As a Candidate for Tumor Immunotherapy, IUBMB Life (2019)
16. Nanoparticles As New Tools for Inhibition of Cancer Angiogenesis, Journal of Cellular Physiology (2018)
17. Glioblastoma Cancer Stem Cell Biology: Potential Theranostic Targets, Drug Resistance Updates (2019)
18. Tumor Immunology, Clinical Immunology (2022)
19. The Pivotal Role of Angiogenesis in a Multi-Scale Modeling of Tumor Growth Exhibiting the Avascular and Vascular Phases, Microvascular Research (2018)
20. The Pro-Tumorigenic Responses in Metastatic Niches: An Immunological Perspective, Clinical and Translational Oncology (2023)