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Fabrication and Evaluation of Cs/Pvp Sponge Containing Platelet-Rich Fibrin As a Wound Healing Accelerator: An in Vitro and in Vivo Study Publisher Pubmed



Tavakoli M1 ; Mirhaj M1 ; Labbaf S1 ; Varshosaz J2 ; Taymori S2 ; Jafarpour F3 ; Salehi S4 ; Abadi SAM5 ; Sepyani A6
Authors
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Authors Affiliations
  1. 1. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  2. 2. Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Iran
  3. 3. Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
  4. 4. Department of Materials Engineering, Islamic Azad University, Najafabad, Iran
  5. 5. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
  6. 6. Department of Tissue Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran

Source: International Journal of Biological Macromolecules Published:2022


Abstract

Despite significant advances in surgery and postoperative care, there are still challenges in the treatment of wounds. In the current study, a freeze-dried chitosan (Cs)/polyvinylpyrrolidone (PVP) sponges containing platelet-rich fibrin (PRF at 1, 1.5 and 2% w/v) for wound dressing application is fabricated and fully characterized. Addition of 1% w/v of PRF to Cs/PVP (CS/PVP/1PRF) sample significantly increased the tensile strength (from 0.147 ± 0.005 to 0.242 ± 0.001 MPa), elastic modulus (from 0.414 ± 0.014 to 0.611 ± 0.022 MPa) and strain at break (from 53.4 ± 0.9 to 61.83 ± 1.17%) compared to Cs sample, and was hence selected as the optimal sample. The antibacterial activity of Cs/PVP/1PRF sponge wound dressing against E. coli and S. aureus was confirmed to be effective. Enzyme-linked immunosorbent assays revealed that the release of both VEGF and PDGF-AB from PRF powder, as well as PDGF-AB from Cs/PVP/1PRF sample was time-independent, but the release of VEGF from Cs/PVP/1PRF sample increased significantly with time. According to MTT and CAM assays, the Cs/PVP/1PRF sample significantly increased proliferation and angiogenic potential, respectively. Furthermore, in vivo studies demonstrated a 97.16 ± 1.55% wound closure for Cs/PVP/1PRF group after 14 days. © 2022 Elsevier B.V.
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