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Preparation of a Biomimetic Bi-Layer Chitosan Wound Dressing Composed of A-Prf/Sponge Layer and L-Arginine/Nanofiber Publisher Pubmed



Mirhaj M1 ; Tavakoli M1 ; Varshosaz J2 ; Labbaf S1 ; Salehi S3 ; Talebi A4 ; Kazemi N3 ; Haghighi V5 ; Alizadeh M6
Authors
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Authors Affiliations
  1. 1. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  2. 2. Department of Pharmaceutics, Novel Drug Delivery Systems Research Centre, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
  4. 4. Department of Pathology, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran
  5. 5. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
  6. 6. Department of Biomedical Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran

Source: Carbohydrate Polymers Published:2022


Abstract

To better mimic the structure of skin tissue, the use of a multi-layered wound dressing has been proposed. In the present study, a sponge-nanofibrous bi-layer dressing is designed. For this purpose, a chitosan/polyethylene glycol (CsPEG) sponge with advanced platelet-rich fibrin (A-PRF) was prepared as the upper layer of wound dressing, and a Cs/L–arginine electrospun nanofiber layer as the bottom layer. After physical, chemical and mechanical evaluations, the release of platelet-derived growth factor-AB (PDGF-AB), vascular endothelial growth factor (VEGF) and L-arginine were investigated. The antibacterial activity, cell viability and attachment of Bi-layer1.5 dressing (CsPEG/1.5A-PRF sponge coated with Cs/0.5 L-arginine nanofibers) were significantly higher than other dressings. Also, Bi-layer1.5 dressing increased the angiogenic potential and accelerated the wound healing, compared to other samples. Given the promising obtained results, the use of Bi-layer1.5 wound dressing with the ability to release growth factors and L-arginine is highly recommended to treat full-thickness wounds. © 2022
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