Evaluation of Cellular Immunogenicity of Recombinant Cytochrome P450 Cyp141 Protein of Mycobacterium Tuberculosis in Human and Mouse Model Publisher Pubmed



Salehi M^{1, 2} ; Abdizadeh R³ ; Pourgheysari B⁴ ; Zamanzad B¹ ; Soltani A¹ ; Gholipour A¹ ; Heidari R¹ Show Affiliations
Source: Biologicals Published:2018

Abstract

Mycobacterium tuberculosis (Mtb) is still considered one of the unsolved problems for the World Health Organization Identifying and selecting an immunogenic antigen capable of generating specific immune responses is generally the goal of all studies being carried out in to designing new vaccines. Accordingly, the present study was conducted to evaluate the immunogenicity of a M. tuberculosis recombinant protein which exist in the regions of the bacterium genome and may be an immunogenic protein. Immunogenicity of purified proteins was measured by PBMC and mouse spleen lymphocytes culturing methods using ELISA after an appropriate amount of time of incubation with Recombinant cytochrome P450 CYP141 protein. Cellular immune responses were determined and compared by measuring IFN-γ and IL4 in human, and mouse groups. The results revealed a high level of IFN-γ in PPD + individuals and the mice immunized with protein and adjuvant. Recombinant cytochrome P450 CYP141 protein proved capable of generating an immune response in mice and people with a history of previous encounters with Mycobacterium tuberculosis bacteria. It, could be considered a tuberculosis vaccine candidate in order to induce a specific effective immune response in both mice and humans. © 2018