Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Anti Her-2 Aptamer Functionalized Gold Nanoparticles of Dasatinib for Targeted Chemo-Radiotherapy in Breast Cancer Cells Publisher Pubmed



Khorshid M1 ; Varshosaz J1 ; Rostami M2 ; Haghiralsadat F3 ; Akbari V4 ; Khorshid P5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmaceutics, Faculty of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Medicinal Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Advanced Medical Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  4. 4. Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Department of Medicinal Chemistry, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Source: Biomaterials Advances Published:2023


Abstract

In the present study, gold nanoparticles functionalized with anti HER-2 aptamer were designed for effective targeted delivery of dasatinib (DSB) to breast cancer cells. Anti HER-2 aptamer attached to porous or plain gold nanoparticles were compared for dasatinib delivery. Activated drug with succinic anhydride and L-cysteine linker was used for conjugation of DSB to gold nanoparticles. The loading efficiency of the activated drug on plain and porous gold nanoparticles was 52 and 68 %, respectively, which was significantly more than the loading of free DSB in gold nanoparticles (1–2.5 %). The anti HER-2 aptamer was conjugated to porous gold nanoparticles loaded with the activated drug. Various characterization techniques such as FESEM, TEM, AFM, zeta potential and ICP-MS were used to confirm the binding of the drug to gold nanoparticles. 1HNMR and FTIR spectroscopic analyses were employed to examine the structural characteristics of the conjugated drug. These analytical techniques confirmed the successful incorporation of succinyl and thiol groups onto the drug molecule. The amount of aptamer binding to different types of gold nanoparticles was obtained from the intensity of the light emitted from the bands observed in electrophoresis gel and due to the presence of porosity in porous gold nanoparticles, the amount of aptamer conjugation on porous gold nanoparticles increased compared to plain ones. Cell cytotoxicity and cellular uptake were evaluated by MTT assay and TEM in BT-474 and MCF-7 cells. Aptamer-functionalized porous gold nanoparticles containing activated dasatinib showed higher cytotoxicity and cellular uptake than modified DSB-loaded nanoparticles and un-activated DSB. The combination of radiation therapy with the modified dasatinib attached to porous gold nanoparticles and aptamer demonstrated a notable reduction in the IC50 values for both the BT-474 and MCF-7 cell lines. Specifically, the IC50 value for the BT-474 cells decreased from 6.95 μM (for unmodified dasatinib) to 2.57 μM, while for the MCF-7 cells, it decreased from 13.97 μM to 8.57 μM. These findings indicate a significant improvement in the efficacy of the modified dasatinib compared to its unmodified counterpart when used in conjunction with radiation therapy. © 2023 Elsevier B.V.
Related Docs
View other Related Docs
1. Advances in Aptamer-Based Drug Delivery Vehicles for Cancer Therapy, Biomaterials Advances (2022)
2. Smart Redox-Sensitive Micelles Based on Chitosan for Dasatinib Delivery in Suppressing Inflammatory Diseases, International Journal of Biological Macromolecules (2023)
3. Recent Advances in Improved Efficacies of Gold Nano-Formulations in Treatment of Skin Cancer: A Systematic Review, Archives of Dermatological Research (2025)
4. In Vitro Investigation of Radiotherapy Along With Gemcitabine Loaded Peg Gold Nanoparticles Against Mcf-7 Breast Cancer Cells, Advanced Biomedical Research (2025)
5. As1411 Aptamer Conjugated Gold Nanoclusters As a Targeted Radiosensitizer for Megavoltage Radiation Therapy of 4T1 Breast Cancer Cells, RSC Advances (2018)
6. Targeted Nanostructured Lipid Carriers for Delivery of Paclitaxel to Cancer Cells: Preparation, Characterization, and Cell Toxicity, Current Drug Delivery (2017)
7. Recent Approaches for Targeted Drug Delivery in Rheumatoid Arthritis Diagnosis and Treatment, Artificial Cells, Nanomedicine and Biotechnology (2018)
8. In Vitro and in Vivo Evaluation of Novel Dtx-Loaded Multifunctional Heparin-Based Polymeric Micelles Targeting Folate Receptors and Endosomes, Recent Patents on Anti-Cancer Drug Discovery (2020)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (33)
Farshid Hassanzadeh (15)
Other Related Docs
9. Synthesis and in Vitro/In Vivo Characterization of Raloxifene Grafted Poly(Styrene Maleic Acid)-Poly(Amide-Ether-Ester-Imide) Micelles for Targeted Delivery of Docetaxel in G Protein-Coupled Estrogen Receptor Breast Cancer, Anti-Cancer Agents in Medicinal Chemistry (2018)
10. Gold Nanoparticles Loaded Into Niosomes: A Novel Approach for Enhanced Antitumor Activity Against Human Ovarian Cancer, Advanced Powder Technology (2021)
11. Au Nanoparticles in the Diagnosis and Treatment of Ovarian Cancer: A New Horizon in the Personalized Medicine, Nanomedicine Research Journal (2022)
12. Supramolecular Self-Assembled Nanogels a New Platform for Anticancer Drug Delivery, Current Pharmaceutical Design (2017)
13. Implementation of Electrochemical Impedance Spectroscopy to Evaluate Her-2 Aptamer Conjugation to Ecoflex® Nanoparticles for Docetaxel Delivery in Breast Cancer Cells, Journal of Applied Electrochemistry (2019)
14. Hyaluronic Acid Targeted Metal Organic Framework Based on Iron (Iii) for Delivery of Platinum Curcumin Cytotoxic Agent to Triple Negative Breast Cancer Cell Line, Applied Organometallic Chemistry (2022)
15. Folated Synperonic-Cholesteryl Hemisuccinate Polymeric Micelles for the Targeted Delivery of Docetaxel in Melanoma, BioMed Research International (2015)
16. Co-Delivery of Dasatinib and Mir-30A by Liposomes Targeting Neuropilin-1 Receptors for Triple-Negative Breast Cancer Therapy, Journal of Materials Chemistry B (2024)
17. As1411 Aptamer-Targeted Gold Nanoclusters Effect on the Enhancement of Radiation Therapy Efficacy in Breast Tumor-Bearing Mice, Nanomedicine (2018)
18. Highly Sensitive Mixed Molecular Nanostructures of Glutamine and Doxorubicin on Gold for Targeted Recognition of A2780 Cancer Cells Using Impedance Spectroscopy and Quartz Crystal Microbalance, Sensors and Actuators, B: Chemical (2021)
19. A Concise Review on Cancer Treatment Methods and Delivery Systems, Journal of Drug Delivery Science and Technology (2019)
20. Plga-Peg-Ra-Based Polymeric Micelles for Tumor Targeted Delivery of Irinotecan, Pharmaceutical Development and Technology (2018)
21. Recent Progress in Cancer Immunotherapy: Application of Nano-Therapeutic Systems, Journal of Drug Delivery Science and Technology (2024)
22. Mesoporous Silica@Chitosan@Gold Nanoparticles As “On/Off” Optical Biosensor and Ph-Sensitive Theranostic Platform Against Cancer, International Journal of Biological Macromolecules (2022)
23. Feasibility of Haloperidol-Anchored Albumin Nanoparticles Loaded With Doxorubicin As Dry Powder Inhaler for Pulmonary Delivery, Pharmaceutical Development and Technology (2015)
24. Chondroitin/Doxorubicin Nanoparticulate Polyelectrolyte Complex for Targeted Delivery to Hepg2 Cells, IET Nanobiotechnology (2017)
25. Technetium-99M Chelator-Free Radiolabeling of Specific Glutamine Tumor Imaging Nanoprobe: In Vitro and in Vivo Evaluations, International Journal of Nanomedicine (2018)
26. Dendrimers As Nanoscale Vectors: Unlocking the Bars of Cancer Therapy, Seminars in Cancer Biology (2022)
27. Investigation of Different Targeting Decorations Effect on the Radiosensitizing Efficacy of Albumin-Stabilized Gold Nanoparticles for Breast Cancer Radiation Therapy, European Journal of Pharmaceutical Sciences (2019)
28. Evaluation of Lipid Nanocarriers in the Form of Sln and Their Application in Drug Delivery, Nanochemistry Research (2023)
29. Anticancer Nano-Delivery Systems Based on Bovine Serum Albumin Nanoparticles: A Critical Review, International Journal of Biological Macromolecules (2021)
30. Docetaxel-Loaded Nanostructured Lipid Carriers Functionalized With Trastuzumab (Herceptin) for Her2-Positive Breast Cancer Cells, Journal of Liposome Research (2018)
31. Pharmacokinetics and in Vitro/In Vivo Antitumor Efficacy of Aptamer-Targeted Ecoflex® Nanoparticles for Docetaxel Delivery in Ovarian Cancer, International Journal of Nanomedicine (2018)
32. Peptide-Based Pegylated Polyurethane Nanoparticles for Paclitaxel Delivery in Hela Cancer Cells: The Art of the Architecture Design in Nanocarriers, Polymer Bulletin (2023)
33. Could Fa-Pg-Spions Act As a Hyperthermia Sensitizing Agent? an in Vitro Study, Journal of Thermal Biology (2018)
34. Cytotoxic Effects of Targeted Galactosylated Pluronic F127/Pluronicl122 Nano-Sized Micelles Loaded With Doxorubicin in Human Hepatocellular Carcinoma Cell Line, Journal of Isfahan Medical School (2013)
35. Evaluation of Thermosensitive Chitosan Hydrogel Containing Gefitinib Loaded Cellulose Acetate Butyrate Nanoparticles in a Subcutaneous Breast Cancer Model, International Journal of Pharmaceutics (2022)
36. An Insight Into Fluorescence and Magnetic Resonance Bioimaging Using a Multifunctional Polyethyleneimine-Passivated Gadocarbon Dots Nanoconstruct Assembled With As1411, Microchimica Acta (2023)
37. Tissue Distribution and Systemic Toxicity Evaluation of Raloxifene Targeted Polymeric Micelles of Poly (Styrene-Maleic Acid)-Poly (Amide- Ether-Ester-Imide)-Poly (Ethylene Glycol) Loaded With Docetaxel in Breast Cancer Bearing Mice, Recent Patents on Anti-Cancer Drug Discovery (2019)
38. Fluorescence Resonance Energy Transfer Monitoring of Ph-Responsive Doxorubicin Release From Carbon Dots/Aptamer Functionalized Magnetic Mesoporous Silica, Nanomedicine (2021)
39. Optical, Physical and Quantum Properties of Gold Nanoparticles and Its Applications in Diagnosis and Treatment of Cancers, Journal of Isfahan Medical School (2015)
40. The Effect of Folic Acid-Targeted Nanocarriers in Ultrasound Imaging-Guided Sonodynamic Therapy of Human Cervical Carcinoma (Hela): In Vitro Study, Koomesh (2023)
41. Nanobodies; New Molecular Instruments With Special Specifications for Targeting, Diagnosis and Treatment of Triple-Negative Breast Cancer, Cancer Cell International (2022)
42. Co-Delivery of Carboplatin and Doxorubicin Using Zif-8 Coated Chitosan-Poly(N-Isopropyl Acrylamide) Nanoparticles Through a Dual Ph/Thermo Responsive Strategy to Breast Cancer Cells, International Journal of Biological Macromolecules (2024)
43. Luteinizing Hormone-Releasing Hormone Targeted Poly(Methyl Vinyl Ether Maleic Acid) Nanoparticles for Doxorubicin Delivery to Mcf-7 Breast Cancer Cells, IET Nanobiotechnology (2016)
44. Hierarchical Multifunctional Graphene Oxide Cancer Nanotheranostics Agent for Synchronous Switchable Fluorescence Imaging and Chemical Therapy, Microchimica Acta (2020)
45. Functionalized Silver and Gold Nanomaterials With Diagnostic and Therapeutic Applications, Pharmaceutics (2022)
46. A Review on Application of Novel Solid Nanostructures in Drug Delivery, Journal of Nano Research (2018)
47. Folate-Targeted Polyacrylamide/Punicic Acid Nanomicelles for Flutamide Delivery in Prostate Cancer: Characterization, in Vitro Biological Evaluation, and Its Dft Study, Recent Patents on Nanotechnology (2020)
48. Recent Advances in Nanomaterials Development for Nanomedicine and Cancer, ACS Applied Bio Materials (2021)
49. Lipid Nanocapsules for Imatinib Delivery: Design, Optimization and Evaluation of Anticancer Activity Against Melanoma Cell Line, Iranian Journal of Pharmaceutical Research (2019)
50. Novel Nanoscale Vanillin Based Covalent Triazine Framework As a Novel Carrier for Sustained Release of Imatinib, Polymers for Advanced Technologies (2023)