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Comparison of Acellular and Cellular Bioactivity of Poly 3-Hydroxybutyrate/Hydroxyapatite Nanocomposite and Poly 3-Hydroxybutyrate Scaffolds Publisher



Saadat A1 ; Behnamghader A2 ; Karbasi S3 ; Abedi D4 ; Soleimani M5 ; Shafiee A6, 7
Authors
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Authors Affiliations
  1. 1. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Biomaterials Group, Materials and Energy Research Center, Tehran 13145-1659, Iran
  3. 3. Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran
  4. 4. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran
  5. 5. Department of Hematology, School of Medicine, Tarbiat Modares University, Tehran, Iran
  6. 6. Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

Source: Biotechnology and Bioprocess Engineering Published:2013


Abstract

Nanocomposites have recently been identified as a useful scaffolding material in tissue engineering applications. Poly (3-hydroxybutyrate)/ hydroxyapatite nanoparticles (P3HB)/(nHA) porous scaffolds were successfully fabricated through a solvent casting and particulate leaching technique. P3HB/nHA and P3HB scaffolds were prepared by the same technique for comparison. The structure of the nanocomposite and P3HB scaffolds was observed by SEM. The Energy Disperssive X-ray Analysis (EDXA, map of Ca) results indicated that HA nanoparticles were homogeneously dispersed in the P3HB matrix. X-ray diffraction (XRD) analysis showed that P3HB and HA coexist in the nanocomposite. Transmission electron microscopy (TEM) images also showed that the particle size of HA was 30 ∼ 40 nm. The porosity of the scaffolds was 84%, and macropores and micropores coexisted and interconnected throughout the scaffolds. Acellular bioactivity experiments showed that more HA crystals formed on the surface of the nanocomposite scaffold than on the P3HB scaffold after 4 weeks immersion in Simulated Body Fluid (SBF). Cell culture experiments demonstrated that the P3HB/nHA nanocomposite scaffold had a better tendency of proliferation and Alkaline Phosphatase (ALP) activity to MG 63 cells than the pure P3HB scaffold. It was found that nHA addition can improve acellular and cellular bioactivity of the P3HB scaffold. © 2013 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
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