Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (Cdpk7) Showed Potential Targets for Toxoplasma Gondii Vaccine Publisher



Taghipour A1 ; Tavakoli S2 ; Sabaghan M3 ; Foroutan M4 ; Majidiani H5 ; Soltani S4 ; Badri M6 ; Ghaffari AD1 ; Soltani S4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  2. 2. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Behbahan Faculty of Medical Sciences, Behbahan, Iran
  4. 4. USERN Office, Abadan University of Medical Sciences, Abadan, Iran
  5. 5. Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran
  6. 6. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

Source: Journal of Parasitology Research Published:2021


Abstract

Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79 D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future. © 2021 Ali Taghipour et al.
Related Docs
View other Related Docs
1. Toxoplasma Gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look Through an in Silico Prism, BioMed Research International (2021)
2. Rhomboid Antigens Are Promising Targets in the Vaccine Development Against Toxoplasma Gondii, EXCLI Journal (2019)
3. Insights Into the Biochemical Features and Immunogenic Epitopes of Common Bradyzoite Markers of the Ubiquitous Toxoplasma Gondii, Infection, Genetics and Evolution (2021)
4. Development of a Chimeric Vaccine Candidate Based on Toxoplasma Gondii Major Surface Antigen 1 and Apicoplast Proteins Using Comprehensive Immunoinformatics Approaches, European Journal of Pharmaceutical Sciences (2021)
5. A Systematic Review of Toxoplasma Gondii Antigens to Find the Best Vaccine Candidates for Immunization, Microbial Pathogenesis (2019)
6. Design of Multivalent-Epitope Vaccine Models Directed Toward the World’S Population Against Hiv-Gag Polyprotein: Reverse Vaccinology and Immunoinformatics, PLoS ONE (2024)
7. Functional and Structural Characterization of Ebola Virus Glycoprotein (1976-2015)-An in Silico Study, International Journal of Biomathematics (2017)
8. Computational Clues of Immunogenic Hotspots in Plasmodium Falciparum Erythrocytic Stage Vaccine Candidate Antigens: In Silico Approach, BioMed Research International (2022)
Experts (# of related papers)
View all Related Experts
Seyed Hossein Hejazi (6)
Hossein Khanahmad (6)
Other Related Docs
9. In Silico Analysis of Synaptonemal Complex Protein 1 (Sycp1) and Acrosin Binding Protein (Acrbp) Antigens to Design Novel Multiepitope Peptide Cancer Vaccine Against Breast Cancer, International Journal of Peptide Research and Therapeutics (2019)
10. Exploring the Cancer-Testis Antigen Boris to Design a Novel Multi-Epitope Vaccine Against Breast Cancer Based on Immunoinformatics Approaches, Journal of Biomolecular Structure and Dynamics (2022)
11. Engineering a Multi-Epitope Vaccine Candidate Against Leishmania Infantum Using Comprehensive Immunoinformatics Methods, Biologia (2022)
12. In-Silico Design of a New Multi-Epitope Vaccine Candidate Against Sars-Cov-2, Acta Virologica (2023)
13. The Probable Association Between Chronic Toxoplasma Gondii Infection and Type 1 and Type 2 Diabetes Mellitus: A Case-Control Study, Interdisciplinary Perspectives on Infectious Diseases (2021)
14. Bioinformatics Design of a Peptide Vaccine Containing Sarcoma Antigen Ny-Sar-35 Epitopes Against Breast Cancer and Evaluation of Its Immunological Function in Balb/C Mouse Model, PLoS ONE (2024)
15. Toxoplasmosis and Symptoms Severity in Patients With Covid-19 in Referral Centers in Northern Iran, Journal of Parasitic Diseases (2023)
16. Multi-Epitope Vaccine Against Cystic Echinococcosis Using Immunodominant Epitopes From Ega31 and Egg1y162 Antigens, Informatics in Medicine Unlocked (2020)
17. Exploring the Out of Sight Antigens of Sars-Cov-2 to Design a Candidate Multi-Epitope Vaccine by Utilizing Immunoinformatics Approaches, Vaccine (2020)
18. Evaluation of Humoral and Cellular Immune Responses Against Vibrio Cholerae Using Oral Immunization by Multi-Epitope-Phage-Based Vaccine, International Immunopharmacology (2024)
19. Novel Sars-Cov2 Poly Epitope Phage-Based Candidate Vaccine and Its Immunogenicity, Research in Pharmaceutical Sciences (2024)
20. In Silico Analysis of Transmembrane Protein 31 (Tmem31)Antigen to Design Novel Multiepitope Peptide and Dna Cancer Vaccines Against Melanoma, Molecular Immunology (2019)
21. Immunoinformatic Approach to the Design of a Novel Multi-Epitope Vaccine Against Leishmania Major Fused to Human Igg-Fc, Research in Pharmaceutical Sciences (2024)
22. Designing a Multi-Epitope Influenza Vaccine: An Immunoinformatics Approach, Scientific Reports (2024)
23. Development of Polyepitopic Immunogenic Contrast Against Hepatitis C Virus 1A-6A Genotype by in Silico Approach, Biomedical and Biotechnology Research Journal (2020)
24. Linear and Conformational B Cell Epitope Prediction of the Her 2 Ecd-Subdomain Iii by in Silico Methods, Asian Pacific Journal of Cancer Prevention (2012)
25. A Lentiviral Vaccine Expressing Kmp11-Haspb Fusion Protein Increases Immune Response to Leishmania Major in Balb/C, Parasitology Research (2018)
26. The Seroprevalence of Anti-Toxoplasma Gondii Igg and Igm Antibodies in Serum Samples Referred to Educational Hospitals of Abadan, Journal of Isfahan Medical School (2022)
27. Evaluation of Th17 Immune Responses of Recombinant Dna Vaccine Encoding Gra14 and Rop13 Genes Against Toxoplasma Gondii in Balb/C Mice, Infection, Genetics and Evolution (2021)
28. Detection of Toxoplasma Gondi in Acute and Chronic Phases of Infection in Immunocompromised Patients and Pregnant Women With Real-Time Pcr Assay Using Taqman Fluorescent Probe, Iranian Journal of Parasitology (2018)
29. Worldwide Prevalence of Human Papillomavirus Among Pregnant Women: A Systematic Review and Meta-Analysis, Reviews in Medical Virology (2023)
30. Sero-Molecular Prevalence of Toxoplasmosis in Hemodialysis and Peritoneal Dialysis Patients in Markazi Province, Iran, Tropical Medicine and Health (2025)
31. Knocking Down the Expression of the Molecular Motors, Myosin A, C and F Genes in Toxoplasma Gondii to Decrease the Parasite Virulence, Experimental Parasitology (2023)
32. Manipulation of Il-10 Gene Expression by Toxoplasma Gondii and Its Products, Medical Journal of the Islamic Republic of Iran (2016)
33. A Systematic Review on the Role of Gra Proteins of Toxoplasma Gondii in Host Immunization, Journal of Microbiological Methods (2019)
34. Evaluation of Toxoplasma Gondii Infection in Western Iran: Seroepidemiology and Risk Factors Analysis, Tropical Medicine and Health (2020)
35. Detection of Toxoplasma Parasitemia by Pcr: Does It Correlate With Igg and Igm Antibody Titers?, Iranian Journal of Immunology (2006)
36. Designing and Characterization of Tregitope-Based Multi-Epitope Vaccine Against Multiple Sclerosis: An Immunoinformatic Approach, Current Drug Safety (2023)
37. Human Anti-Toxoplasma Antibodies Attach Strongly to Breast Cancer Cells, International Journal of Cancer Management (2023)
38. High Occurrence of Toxoplasma Gondii Infection Among Blood Donors in Ardabil Province As Main Focus of Zoonotic Visceral Leishmaniosis, Northwestern Iran, Annals of Parasitology (2021)