Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Systemic Administration of Ghrelin Did Not Restore Angiogenesis in Hindlimb Ischemia in Control and Diet-Induced Obese Mice Publisher Pubmed



Tahergorabi Z1 ; Khazaei M2 ; Rashidi B3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Physiology, Birjand University of Medical Sciences, Birjand, Iran
  2. 2. Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  3. 3. Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Bratislava Medical Journal Published:2015


Abstract

Background: Ghrelin is a novel growth hormone releasing peptide that mainly regulates food intake and energy homeostasis, however, recently, it is indicated that it may be closely related with physiological and/ or pathological angiogenesis. Objectives: The objective of the present study was to evaluate the effect of systemic ghrelin administration on angiogenesis in hindlimb ischemia in normal and diet-induced obese mice. Methods: 24 male C57BL/6 mice were fed with high-fat diet (HFD) or standard for 14 weeks. Then, the mice underwent unilateral hindlimb ischemia. Next, each group was divided into the two subgroups: treatment with ghrelin (100μg/kg, twice daily, Sc) or without treatment. After 10 days, the animals were sacrificed, blood samples were taken and the gastrocnemius muscles removed. Results: There was no significant difference in capillary/fiber ratio in hind limb ischemia between obese and control groups. Administration of ghrelin reduced serum nitric oxide (NO) and leptin and increased vascular endothelial growth factor (VEGF) concentrations in obese mice, however, did not change the capillary/fiber ratio in ischemic legs. Conclusion: Systemic administration of ghrelin did not restore angiogenesis in hindlimb ischemia in control and diet-induced obese mice.
Related Docs
View other Related Docs
1. Systemic Ghrelin Administration Alters Serum Biomarkers of Angiogenesis in Diet-Induced Obese Mice, International Journal of Peptides (2013)
2. Ghrelin Does Not Modulate Angiogenesis in Matrigel Plug in Normal and Diet-Induced Obese Mice, Journal of Research in Medical Sciences (2013)
3. Ghrelin Does Not Alter Aortic Intima-Media Thickness and Adipose Tissue Characteristics in Control and Obese Mice, Iranian Journal of Basic Medical Sciences (2013)
4. Obesity and Angiogenesis, Journal of Isfahan Medical School (2012)
5. The Relationship Between Inflammatory Markers, Angiogenesis, and Obesity, ARYA Atherosclerosis (2013)
6. Correlations Between Serum Leptin and Nitric Oxide Concentrations in Obese and Control Balb/C Mice, Journal of Isfahan Medical School (2013)
7. Restoration of Angiogenesis by Enalapril in Diabetic Hindlimb Ischemc Rats, Biomedical Papers (2011)
8. Pan-Ppar Agonist, Bezafibrate, Restores Angiogenesis in Hindlimb Ischemia in Normal and Diabetic Rats, International Journal of Peptides (2012)
Experts (# of related papers)
View all Related Experts
Shaghayegh Haghjooy Javanmard (7)
Bahman Rashidi (7)
Other Related Docs
9. Short- and Long-Term Relationships of Serum Ghrelin With Changes in Body Composition and the Metabolic Syndrome in Prepubescent Obese Children Following Two Different Weight Loss Programmes, Clinical Endocrinology (2008)
10. Role of Peroxisome Proliferator-Activated Receptor Β Agonist on Angiogenesis in Hindlimb Ischemic Diabetic Rats, Journal of Diabetes and its Complications (2012)
11. Effects of Modified-Paleo and Moderate-Carbohydrate Diets on Body Composition, Serum Levels of Hepatokines and Adipocytokines, and Flow Cytometric Analysis of Endothelial Microparticles in Adults With Metabolic Syndrome: A Study Protocol for a Randomized Clinical Trial, Trials (2021)
12. Prognostic Value of Circulating Macrophage Inhibitory Cytokine 1-Growth Differentiation Factor 15 (Mic-1/Gdf15) in Obesity: Relation With Vascular Endothelial Growth Factor (Vegf) and Markers of Oxidative Stress, Nutrition and Health (2023)
13. Effects of Chronic Scheduled–Caloric Dietary on Ghrelin Secretion and Food Intake in Adult Male Rats, Physiology and Pharmacology (Iran) (2019)
14. Sustained Feeding of a Diet High in Fat Resulted in a Decline in the Liver's Insulin-Degrading Enzyme Levels in Association With the Induction of Oxidative and Endoplasmic Reticulum Stress in Adult Male Rats: Evaluation of 4-Phenylbutyric Acid, Heliyon (2024)
15. Long-Term Gaba Supplementation Regulates Diabetic Gastroenteropathy Through Gaba Receptor/Trypsin-1/Pars/Akt/Cox-2 Axis, Doklady Biochemistry and Biophysics (2024)
16. Effect of Vanadyl Sulfate on Fructose-Induced Insulin Resistance Rat, Iranian Biomedical Journal (2003)
17. Can Flaxseed Supplementation Affect Circulating Adipokines in Adults? an Updated Systematic Review and Meta-Analysis of Randomized Controlled Trials, Frontiers in Nutrition (2023)
18. Effect of Blockade of Neuropeptide Y Receptor on Aortic Intima-Media Thickness and Adipose Tissue Characteristics in Normal and Obese Mice, Iranian Journal of Basic Medical Sciences (2015)
19. Gastroileostomy for Controlling Body Weight and Lipid Profile: An Experimental Rat Model, Obesity Medicine (2019)
20. Effect of L-Arginine and L-Name on Coronary Angiogenesis in Male Diabetic Rats, Journal of Research in Medical Sciences (2012)
21. Assessment of Endothelial Function in Iranian Healthy Obese Patients After Bariatric Surgery, Advanced Biomedical Research (2022)
22. Role of Fenofibrate in Restoring Angiogenesis in Diabetic and Control Hind Limb Ischemic Rats, General Physiology and Biophysics (2012)
23. The Effects of L-Arginine (The Precursor of Nitric Oxide) and L-Name (Nitric Oxide Synthase Inhibitor) on Coronary Angiogenesis in Normal Rats, Journal of Isfahan Medical School (2011)
24. The Effect of Aspirin on Serum Vascular Endothelial Growth Factor and Nitric Oxide Concentration in High-Cholesterol Fed Rabbits, ARYA Atherosclerosis (2007)
25. Evaluation of Fat Cell's Size, Number and Their Mediators in Ischemic Heart Disease Patients, Journal of Isfahan Medical School (2010)
26. Involvement of Cholinergic Pathway in Energy Homeostasis Via Ventromedial Hypothalamic D2 Receptors, Journal of Isfahan Medical School (2019)
27. Effects of Dapt on Serum Vascular Endothelial Growth Factor and Its Soluble Receptor in Obese Balb/C Mice, Journal of Isfahan Medical School (2013)
28. Leptin Promotes Melanoma Tumor Growth in Mice Related to Increasing Circulating Endothelial Progenitor Cells Numbers and Plasma No Production, Journal of Experimental and Clinical Cancer Research (2011)
29. Association of the Serum Apelin, But Not Ghrelin, With the Presence and Severity of Coronary Artery Disease, Indian Heart Journal (2021)
30. Von Willebrand Factor, C-Reactive Protein, Nitric Oxide, and Vascular Endothelial Growth Factor in a Dietary Reversal Model of Hypercholesterolemia in Rabbit., Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia (2008)
31. The Effects of Aerobic Exercise on Liver Function, Insulin Resistance, and Lipid Profiles in Prediabetic and Type 2 Diabetic Mice, Physiology and Behavior (2023)
32. Inducible Nitric Oxide Synthase Modulates Angiogenesis in Ischemic Hindlimb of Rat, Biomedical Papers (2009)
33. Limbs’ Postischemic Revascularization Is Not Improved by Losartan Treatment in Diabetic Rats, Endocrine Regulations (2014)
34. Coronary Angiogenesis During Experimental Hypertension: Is It Reversible?, Journal of Research in Medical Sciences (2011)
35. Growth Hormone Response to L-Arginine Alone and Combined With Different Doses of Growth Hormone-Releasing Hormone: A Systematic Review and Meta-Analysis, International Journal of Endocrinology (2022)
36. Possible Involvement of the Dopamine D2 Receptors of Ventromedial Hypothalamus in the Control of Free- and Scheduled-Feeding and Plasma Ghrelin Level in Rat, Journal of Complementary and Integrative Medicine (2021)
37. Rosiglitazone Enhances Neovascularization in Diabetic Rat Ischemic Hindlimb Model, Biomedical Papers (2012)
38. Relationship Between Angptl3 and Vo2max, Body Composition and Markers of Metabolic Syndrome and Effect of Interval Training on These Variables in Overweight and Obese Women, Journal of Mazandaran University of Medical Sciences (2021)
39. The Effects of Resistance Training on Plasma Angiogenic Factors in Normal Rats, Journal of Isfahan Medical School (2012)
40. The Effect of Hypercholesterolemia on Serum Vascular Endothelial Growth Factor and Nitrite Concentrations in Early Stage of Atherosclerosis in Rabbits, Pakistan Journal of Nutrition (2009)
41. Effects of a Moderately-Restricted Carbohydrate Diet on Cardiovascular Risk Factors Among Women With Metabolic Syndrome, Journal of Isfahan Medical School (2012)
42. The Impacts of Gastroileostomy Rat Model on Glucagon-Like Peptide-1: A Promising Model to Control Type 2 Diabetes Mellitus, Obesity Surgery (2018)
43. The Effect of Moderate Substitution of Dietary Carbohydrates by Fats on Serum Levels of Adipocytokines, Inflammatory Indices, and Biomarkers of Endothelial Function Amongwomen With Metabolic Syndrome, Journal of Zanjan University of Medical Sciences and Health Services (2012)
44. Antiobesity Effects of Seedlac and Shellac in Rats Fed With a High-Fat Diet, Research in Pharmaceutical Sciences (2020)
45. Vitamin C Protects Against Chronic Social Isolation Stress-Induced Weight Gain and Depressive-Like Behavior in Adult Male Rats, Endocrine Regulations (2020)
46. The Influence of Fasting and Energy-Restricted Diets on Leptin and Adiponectin Levels in Humans: A Systematic Review and Meta-Analysis, Clinical Nutrition (2021)
47. Suppressive Impact of Anethum Graveolens Consumption on Biochemical Risk Factors of Atherosclerosis in Hypercholesterolemic Rabbits, International Journal of Preventive Medicine (2013)
48. Gamma-Secretase Inhibitor Does Not Modulate Angiogenesis in Colon Adenocarcinoma in Obese Mice, Bratislava Medical Journal (2015)
49. Angiopoietin-Like Proteins 2 and 3 in Children and Adolescents With Obesity and Their Relationship With Hypertension and Metabolic Syndrome, International Journal of Hypertension (2021)