Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Lipid Nanocapsules for Imatinib Delivery: Design, Optimization and Evaluation of Anticancer Activity Against Melanoma Cell Line Publisher



Molaahmadi MR1, 2 ; Varshosaz J1, 2 ; Taymouri S1, 2 ; Akbari V3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Iranian Journal of Pharmaceutical Research Published:2019


Abstract

Lipid nanocapsules (LNCs) represent a stable, biocompatible and worthwhile drug delivery system, demonstrating significant potential as gene/drug delivery platforms for cancer therapy. Imatinib, a potent tyrosine kinase inhibitor, has revolutionized the therapy of malignancies resulting from abnormal tyrosine kinase activity. However, its Clinical effectiveness in cancer treatment is hampered by its off-target side effects. In this study, we have investigated the potential benefits of LNCs as a novel drug delivery vehicle for imatinib with a view to improve drug efficacy. LNC formulations were prepared by phase-inversion temperature method and the effects of various formulation variables were assessed using full factorial design. The cytotoxicity and cellular uptake of optimized formulation were investigated against B16F10 melanoma cell line. Analysis of result by Design-Expert® software indicated that Solutol HS15 percent was the most effective parameter on the encapsulation efficiency, particle size, zeta potential, and release efficiency of LNCs. The optimized formulation revealed a particle size of 38.96 ± 0.84 nm, encapsulation efficiency of 99.17 ± 0.086%, zeta potential of-21.5 ± 0.61 mV, release efficiency of 60.03 ± 4.29, and polydispersity index of 0.24 ± 0.02. The imatinib loaded LNCs showed no hemolysis activity. Fluorescent microscopy test showed that the cellular uptake of LNCs was time dependent and density of fluorescent signals increased with time in cells. The in-vitro cytotoxicity study indicated that imatinib kept its pharmacological activity when loaded into LNCs. These results introduced imatinib loaded LNCs as a promising candidate for further investigation in cancer therapy. © 2019, Iranian Journal of Pharmaceutical Research. All rights reserved.
Related Docs
View other Related Docs
1. Efavirenz Oral Delivery Via Lipid Nanocapsules: Formulation, Optimisation, and Ex-Vivo Gut Permeation Study, IET Nanobiotechnology (2018)
2. Preparation and Characterization of Dry Powder Containing Sunitinib Loaded Phbv Nanoparticles for Enhanced Pulmonary Delivery, Journal of Drug Delivery Science and Technology (2020)
3. Enhanced Solubility and Permeability of Naringenin Across Non-Everted Sacs of Rat Small Intestine by Lipid Nanocapsules, Recent Patents on Nanotechnology (2021)
4. Covalent Triazine-Based Polyimine Framework As a Biocompatible Ph-Dependent Sustained-Release Nanocarrier for Sorafenib: An in Vitro Approach, Journal of Molecular Liquids (2020)
5. Encapsulation of Imatinib in Targeted Kit-5 Nanoparticles for Reducing Its Cardiotoxicity and Hepatotoxicity, Anti-Cancer Agents in Medicinal Chemistry (2020)
6. Sorafenib Loaded Pluronic F127-Lithocholic Acid Micelles for Prostate Cancer Therapy: Formulation, Optimization, and in Vitro Evaluation Against Lncap Cells, International Journal of Polymeric Materials and Polymeric Biomaterials (2020)
7. Biological Effects of Letrozole-Loaded Lipid Nanocapsules on Mcf-7 Cell Line, Journal of Isfahan Medical School (2013)
8. Formulation and Characterization of Cellulose Acetate Butyrate Nanoparticles Loaded With Nevirapine for Hiv Treatment, Journal of Drug Delivery Science and Technology (2018)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (95)
Farshid Hassanzadeh (30)
Other Related Docs
9. Optimisation of Processing Variables Effective on Self-Assembly of Folate Targeted Synpronic-Based Micelles for Docetaxel Delivery in Melanoma Cells, IET Nanobiotechnology (2015)
10. Folated Synperonic-Cholesteryl Hemisuccinate Polymeric Micelles for the Targeted Delivery of Docetaxel in Melanoma, BioMed Research International (2015)
11. Trastuzumab-Conjugated Nanoparticles Composed of Poly(Butylene Adipate-Co-Butylene Terephthalate) Prepared by Electrospraying Technique for Targeted Delivery of Docetaxel, IET Nanobiotechnology (2019)
12. Targeting Etoposide to Acute Myelogenous Leukaemia Cells Using Nanostructured Lipid Carriers Coated With Transferrin, Nanotechnology (2012)
13. Pulmonary Delivery of Docetaxel and Celecoxib by Plga Porous Microparticles for Their Synergistic Effects Against Lung Cancer, Anti-Cancer Agents in Medicinal Chemistry (2022)
14. Uptake of Etoposide in Ct-26 Cells of Colorectal Cancer Using Folate Targeted Dextran Stearate Polymeric Micelles, BioMed Research International (2014)
15. Self-Assembly Micelles With Lipid Core of Cholesterol for Docetaxel Delivery to B16f10 Melanoma and Hepg2 Cells, Journal of Liposome Research (2015)
16. Novel Nanoscale Vanillin Based Covalent Triazine Framework As a Novel Carrier for Sustained Release of Imatinib, Polymers for Advanced Technologies (2023)
17. Targeted Nanostructured Lipid Carriers for Delivery of Paclitaxel to Cancer Cells: Preparation, Characterization, and Cell Toxicity, Current Drug Delivery (2017)
18. Lycopene Nanodelivery Systems; Recent Advances, Trends in Food Science and Technology (2022)
19. Co-Delivery of Interferon Regulatory Factor 5 (Irf5) Sirna and Dasatinib by a Disulfide Bond Bearing Polymeric Carrier for Enhanced Anti-Inflammatory Effects, International Journal of Biological Macromolecules (2024)
20. Redox Sensitive Polysaccharide Based Nanoparticles for Improved Cancer Treatment: A Comprehensive Review, Current Pharmaceutical Design (2018)
21. Pegylated Trimethylchitosan Emulsomes Conjugated to Octreotide for Targeted Delivery of Sorafenib to Hepatocellular Carcinoma Cells of Hepg2, Journal of Liposome Research (2019)
22. Preparation, Characterization, and in Vitro Evaluation of Bleomycin-Containing Nanoliposomes, Chemical Biology and Drug Design (2017)
23. Galactosylated Nanostructured Lipid Carriers for Delivery of 5-Fu to Hepatocellular Carcinoma, Journal of Liposome Research (2012)
24. Preparation and Evaluation of Targeted Albumin Lipid Nanoparticles With Lactobionic Acid for Targeted Drug Delivery of Sorafenib in Hepatocellular Carcinoma, Journal of Drug Delivery Science and Technology (2022)
25. Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded With Doxorubicin on Human Hepatoma Cells, Journal of Isfahan Medical School (2013)
26. Co-Delivery of Carboplatin and Doxorubicin Using Zif-8 Coated Chitosan-Poly(N-Isopropyl Acrylamide) Nanoparticles Through a Dual Ph/Thermo Responsive Strategy to Breast Cancer Cells, International Journal of Biological Macromolecules (2024)
27. Biotin Decorated Sunitinib Loaded Nanostructured Lipid Carriers for Tumor Targeted Chemotherapy of Lung Cancer, Journal of Drug Delivery Science and Technology (2019)
28. Development of Dry Powder Inhaler Containing Tadalafil-Loaded Plga Nanoparticles, Research in Pharmaceutical Sciences (2017)
29. Development and Optimization of Transferrin-Conjugated Nanostructured Lipid Carriers for Brain Delivery of Paclitaxel Using Box–Behnken Design, Pharmaceutical Development and Technology (2017)
30. A Concise Review on Cancer Treatment Methods and Delivery Systems, Journal of Drug Delivery Science and Technology (2019)
31. Doxorubicin Hydrochloride - Loaded Electrospun Chitosan/Cobalt Ferrite/Titanium Oxide Nanofibers for Hyperthermic Tumor Cell Treatment and Controlled Drug Release, International Journal of Biological Macromolecules (2018)
32. Mesoporous Silica@Chitosan@Gold Nanoparticles As “On/Off” Optical Biosensor and Ph-Sensitive Theranostic Platform Against Cancer, International Journal of Biological Macromolecules (2022)
33. Feasibility of Haloperidol-Anchored Albumin Nanoparticles Loaded With Doxorubicin As Dry Powder Inhaler for Pulmonary Delivery, Pharmaceutical Development and Technology (2015)
34. Genetically Engineered Viral Vectors and Organic-Based Non-Viral Nanocarriers for Drug Delivery Applications, Advanced Healthcare Materials (2022)
35. Dendrimers As Nanoscale Vectors: Unlocking the Bars of Cancer Therapy, Seminars in Cancer Biology (2022)
36. Preparation and Evaluation of Imatinib Loaded Transfersomal Gel for the Treatment of Rheumatoid Arthritis, Iranian Journal of Pharmaceutical Research (2021)
37. Poly(Glycerol Sebacate) Nanoparticles for Ocular Delivery of Sunitinib: Physicochemical, Cytotoxic and Allergic Studies, IET Nanobiotechnology (2019)
38. Sirna Delivery Improvement by Co-Formulation of Different Modified Polymers in Erythroleukemic Cell Line K562, Iranian Journal of Basic Medical Sciences (2013)
39. Biotin-Encoded Pullulan-Retinoic Acid Engineered Nanomicelles: Preparation, Optimization and in Vitro Cytotoxicity Assessment in Mcf-7 Cells, Indian Journal of Pharmaceutical Sciences (2016)
40. 3D Printed Tablets Containing Oxaliplatin Loaded Alginate Nanoparticles for Colon Cancer Targeted Delivery. an in Vitro/In Vivo Study, International Journal of Biological Macromolecules (2022)
41. Nanoparticles for Targeted Delivery of Therapeutics and Small Interfering Rnas in Hepatocellular Carcinoma, World Journal of Gastroenterology (2015)
42. Ciprofloxacin Loaded Alginate/Chitosan and Solid Lipid Nanoparticles, Preparation, and Characterization, Journal of Dispersion Science and Technology (2012)
43. Physicochemical, Pharmacodynamic and Pharmacokinetic Characterization of Soluplus Stabilized Nanosuspension of Tacrolimus, Current Drug Delivery (2017)
44. Magnetic Polyvinyl Caprolactam–Polyvinyl Acetate–Polyethylene Glycol Micelles for Docetaxel Delivery in Breast Cancer: An in Vitro Study on Two Cell Lines of Breast Cancer, Pharmaceutical Development and Technology (2017)
45. Boron Phenyl Alanine Targeted Chitosan–Pnipaam Core–Shell Thermo-Responsive Nanoparticles: Boosting Drug Delivery to Glioblastoma in Bnct, Drug Development and Industrial Pharmacy (2021)
46. Multifunctional and Stimuli-Responsive Liposomes in Hepatocellular Carcinoma Diagnosis and Therapy, Translational Oncology (2024)
47. Camouflaged Liposomes by 11A4-Nanobody for Co-Delivery of Cisplatin and Nitroxoline in Breast Cancer Tumors: An in Vitro/In Vivo Study, Journal of Drug Delivery Science and Technology (2022)
48. Pegylated Multifunctional Ph-Responsive Targeted Polymeric Micelles for Ovarian Cancer Therapy: Synthesis, Characterization and Pharmacokinetic Study, International Journal of Polymeric Materials and Polymeric Biomaterials (2021)
49. Evaluation of Lipid Nanocarriers in the Form of Sln and Their Application in Drug Delivery, Nanochemistry Research (2023)
50. Anticancer Nano-Delivery Systems Based on Bovine Serum Albumin Nanoparticles: A Critical Review, International Journal of Biological Macromolecules (2021)