Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Transcriptomic Evaluation of Cbfa1 Gene During Osteogenic Differentiation of Adipose Derived Mesenchymal Stem Cells



Daraei A1 ; Ghafarizadeh AA2 ; Hashemibeni B3 ; Salehi R3 ; Salehi M4 ; Bahrambeigi V5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Iranian Academic Center for Education Culture and Research, Markazi Branch, Arak, Iran
  3. 3. Department of Anatomy and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Biostatistics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Department of Anatomy and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2013

Abstract

Background: Bone is a tissue with high repair capacity; but, this ability dramatically is limited during irrecoverable bone and skeletal defects. Stem cell therapy is a promising approach for construction of bone tissue. Mesenchymal stem cells (MSCs) have been introduced as basic tools for bone tissue generation in tissue engineering. Core binding factor alpha 1 (Cbfa1) is one of the main genes involved in osteogenesis. Since relative expression of Cbfa1 in differentiated osteoblasts from adipose derived stem cells is unknown, this study aimed to evaluate the gene expression pattern of Cbfa1 in adipose derived differentiated osteoblasts in 4 different time intervals. Methods: Real-time PCR was used for studying the gene expression of Cbfa1 in human normal osteoblasts and adipose derived osteogenic osteoblasts at the days 7, 14, 21 and 28. Findings: There was a progressive increase in Cbfa1 expression over the differentiation period of adipose derived mesenchymal stem cells (ADSCs) from day 7 to day 28. Differentiated osteoblasts expressed Cbfa1 lower than native osteoblasts (P < 0.05). Conclusion: ADSCs are an attractive source for engineering a new bone tissue. According to our results, adipose derived differentiated osteoblasts express candidate gene at least on day 7 of culture. Our study indicates that longer treatment of ADSCs in osteogenic medium confer better osteoblastic properties to these cells.
Related Docs
View other Related Docs
1. The Expression of Osteogenic Markers in Differentiated Osteoblasts From Adipose-Derived Stem Cells in Pellet Culture and Monolayer Systems, Journal of Isfahan Medical School (2012)
2. Bone Repair With Differentiated Osteoblasts From Adipose-Derived Stem Cells in Hydroxyapatite/Tricalcium Phosphate in Vivo, International Journal of Preventive Medicine (2016)
3. Effect of Transforming Growth Factor-Β3 and Bone Morphogenetic Protein-6 Growth Factors on Chondrogenic Differentiation of Adipose-Derived Stem Cells in Alginate Scaffold, Journal of Isfahan Medical School (2010)
4. An Animal Model Study for Repair of Tracheal Defects With Autologous Stem Cells and Differentiated Chondrocytes From Adipose-Derived Stem Cells, Journal of Pediatric Surgery (2012)
5. Chondrogenic Differentiation of Human Scalp Adipose-Derived Stem Cells in Polycaprolactone Scaffold and Using Freeze Thaw Freeze Method, Journal of Cellular Physiology (2018)
6. Combined Effect of Retinoic Acid and Calcium on the in Vitro Differentiation of Human Adipose-Derived Stem Cells to Adipocytes, Archives of Physiology and Biochemistry (2018)
7. Adipose-Derived Stem Cells Combined With Beta-Tricalcium Phosphate: A Novel Possible Strategy for Periodontal Defects Regeneration, Medical Hypotheses (2014)
8. The Role of Calcium in Differentiation of Human Adipose-Derived Stem Cells to Adipocytes, Molecular Biotechnology (2018)
Experts (# of related papers)
View all Related Experts
Batool Hashemibeni (28)
Mohammad Kazemi (14)
Other Related Docs
9. An Overview of Neural Differentiation Potential of Human Adipose Derived Stem Cells, Stem Cell Reviews and Reports (2016)
10. Comparison Between the Effect of Kartogenin and Tgfβ3 on Chondrogenesis of Human Adipose- Derived Stem Cells in Fibrin Scaffold, Bratislava Medical Journal (2017)
11. Comparison of Aggrecan Gene Expression in Chondrogenesis of Adipose-Derived Stem Cells in Pellet and Micromass Culture Systems, Tehran University Medical Journal (2018)
12. Evaluation of Pomegranate Seed Extract and Tgf-Β3 on Chondrogenic Differentiation of Human Adipose-Derived Stem Cells, Journal of Applied Biotechnology Reports (2022)
13. Utilizing Stem Cells in Reconstructive Treatments for Sports Injuries: An Innovative Approach, Tissue and Cell (2023)
14. Comparing the Effects of Transforming Growth Factor Beta1 (Tgf-ß1) and Piascledine on the Expression of Collagen Ii, X and Aggrecan Genes in Chondrogenesis of Human Adipose-Derived Stem Cells in Fibrin Alginate Composite Scaffold, Journal of Isfahan Medical School (2016)
15. Differentiation of Human Adipose-Derived Stem Cells Toward Neural Cells Using Their Conditioned Medium, Journal of Isfahan Medical School (2017)
16. The Beneficial Effect of Encapsulated Human Adipose-Derived Stem Cells in Alginate Hydrogel on Neural Differentiation, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2014)
17. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
18. The Effect of Pomegranate Extract on Producing Type Ii Collagen in Differentiation of Adipose-Derived Stem Cells Into Chondrocytes, Journal of Isfahan Medical School (2018)
19. Evaluation of the Osteogenic Effect of Apigenin on Human Mesenchymal Stem Cells by Inhibiting Inflammation Through Modulation of Nf-Κb/Iκbα, Research in Pharmaceutical Sciences (2022)
20. Investigation and Comparison of the Effect of Tgf-Β3, Kartogenin and Avocado/Soybean Unsaponifiables on the In-Vitro and In-Vivo Chondrogenesis of Human Adipose-Derived Stem Cells on Fibrin Scaffold, Iranian Journal of Pharmaceutical Research (2021)
21. Induction of Neuron-Like Cells From Adipose Derived Stem Cells in Alginate Hydrogel, Using Neurospheres Formation, Journal of Isfahan Medical School (2014)
22. The Effect of High Frequency Electric Field on Enhancement of Chondrogenesis in Human Adipose-Derived Stem Cells, Iranian Journal of Basic Medical Sciences (2014)
23. Effects of Avocado/Soybean on the Chondrogenesis of Human Adipose-Derived Stem Cells Cultured on Polylactic-Co-Glycolic Acid/Fibrin Hybrid Scaffold, Journal of Applied Biotechnology Reports (2019)
24. A Comparison of Tissue-Engineered Bone From Adipose-Derived Stem Cell With Autogenous Bone Repair in Maxillary Alveolar Cleft Model in Dogs, International Journal of Oral and Maxillofacial Surgery (2013)
25. Expression of Hsa-Mir-204, Runx2, Pparγ, and Bcl2 in Bone Marrow Derived Mesenchymal Stem Cells From, Cell Journal (2017)
26. Evaluation of the Mechanical Characteristics of Plga and Plga/Fibrin Scaffolds in Providing an Appropriate Environment for Viability and Growth of Human Adipose-Derived Stem Cells, Eurasian Journal of Analytical Chemistry (2018)
27. Comparison Between Chondrogenic Markers of Differentiated Chondrocytes From Adipose Derived Stem Cells and Articular Chondrocytes in Vitro, Iranian Journal of Basic Medical Sciences (2013)
28. Comparative Study of Microtubule-Associated Protein-2 and Glial Fibrillary Acidic Proteins During Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells, International Journal of Preventive Medicine (2014)
29. Impact of Fibrin on the Chondrogenic Avocado Soybean Unsaponifiables on Poly (Lactic-Co-Glycolic) Acid Scaffold, Biointerface Research in Applied Chemistry (2021)
30. Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold, International Journal of Preventive Medicine (2014)
31. Comparison of Cd44 and Cd14 Surface Markers in Adipose-Derived Stem Cells and Differentiated Chondrocytes During Chondrogenesis, Journal of Isfahan Medical School (2014)
32. Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells With Tgf-Β3 in Pellet Culture System, Iranian Journal of Basic Medical Sciences (2008)
33. Isolation and Identification of Mesenchymal and Neural Crest Characteristics of Dental Pulp Derived Stem Cells, Koomesh (2015)
34. Effect of T3 Hormone on Neural Differentiation of Human Adipose Derived Stem Cells, Cell Biochemistry and Function (2014)
35. Effect of Piasclidin on Induction of Chondrogenesis by Human Adipose-Derived Stem Cells in Fibrin Scaffold, Journal of Isfahan Medical School (2016)
36. An Animal Model Study for Bone Repair With Encapsulated Differentiated Osteoblasts From Adipose-Derived Stem Cells in Alginate, Iranian Journal of Basic Medical Sciences (2014)
37. Effect of Hypoxia on Mir-21 and Mir-130A Expression in Murine Adipose-Derived Mesenchymal Stem Cells in Primary and Immortality Phases, Journal of Isfahan Medical School (2016)
38. Comparison of Fibrin and Plga/Fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin, Journal of Kerman University of Medical Sciences (2020)
39. Effects of Fgf-2 on Human Adipose Tissue Derived Adult Stem Cells Morphology and Chondrogenesis Enhancement in Transwell Culture, Biochemical and Biophysical Research Communications (2012)
40. Isolation and Differentiation of Adipose-Derived Stem Cells Into Odontoblast-Like Cells: A Preliminary in Vitro Study, Cell Journal (2021)
41. Comparison of the Efficacy of Piascledine and Transforming Growth Factor Β1 on Chondrogenic Differentiation of Human Adipose-Derived Stem Cells in Fibrin and Fibrin-Alginate Scaffolds, Iranian Journal of Basic Medical Sciences (2018)
42. Comparison of Phenotypic Characterization Between Differentiated Osteoblasts From Stem Cells and Calvaria Osteoblasts in Vitro, International Journal of Preventive Medicine (2013)
43. Comparing Brain-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor Secretion of Induced Neurotrophic Factor Secreting Cells From Human Adipose and Bone Marrow-Derived Stem Cells, Development Growth and Differentiation (2013)
44. Comparison of Autogenous Bone Graft and Tissue-Engineered Bone Graft in Alveolar Cleft Defects in Canine Animal Models Using Digital Radiography, Indian Journal of Dental Research (2020)
45. Impact of Ifn-Β and Lif Overexpression on Human Adipose-Derived Stem Cells Properties, Journal of Cellular Physiology (2020)
46. Application of Hanging Drop Culture for Retinal Precursor-Like Cells Differentiation of Human Adipose-Derived Stem Cells Using Small Molecules, Journal of Molecular Neuroscience (2019)
47. Non-Viral Gene Transfer Into Murine Adipose Derived Mesenchymal Stem Cells: A Comparative Study of Different Non-Viral Methods, Gene Therapy and Molecular Biology (2013)
48. Cell Viability and Neural Markers Expression of Human Adipose-Derived Stem Cells After Neural Induction in Alginate Tridimensional Cell Culture, Journal of Isfahan Medical School (2015)
49. Comparison of Two Protocols for Induction of Differentiation of Human Adipose Derived Stem Cells Into Oligodendrocyte Progenitor Cells, Scientific Journal of Kurdistan University of Medical Sciences (2017)
50. Investigating the Effect of Human Adipose Stem Cell-Derived Exosomes on Survival and Neural Differentiation of Pc12 Cells, Journal of Isfahan Medical School (2019)